Abstract: To sort out drought resistance strong barley varieties and in order to find out β-amylase activity influence on barely drought resistance. Under the drought stress simulated with polyethylene glycol (PEG-6000) by nine malting barley of different amylase activity, to evaluate testing varieties of drought resistance based on the determination of seed germination rate and germination potential and to conduct correlation analysis concerning the β-amylase activity and the germination rate under different concentrations of PEG. Results showed that, it was the significant positive correlation at 0.01 level when PEG leve1 was 15%. Barley seed germination rate was declined with the increasing of drought stress. The effect of 5% PEG was less to barley seed germination 10% and 15% PEG significant inhibition barley seed germination. Barley individual varieties stopped germination when PEG leve1 was under 20%. Among process samples: ‘Humai16’ showed good drought resistance comprehensively. Secondly, it was Α7-30, ‘Han98-58’ and ‘Yan95137’ were the worst. 10% of PEG drought stress concentration can be used as appropriate stress concentrations of barley germination period to judge the barley drought resistance, β-amylase activity can be used as one of barley early screening of drought resistance index.