Abstract: In order to ensure the creatures safe living through winter and summer by scientific regulation of water temperature of fishpond, water temperatures of different vertical layers of fishpond and the shore air temperature were researched at different time scales from March 2011 to February 2012 in Wuhan. The results showed that diurnal change trend of water temperatures at different depths of the fishpond was in consistent with that of the shore air temperature which had relatively smaller amplitude. The amplitude of air temperature and water temperature of each layer under clear weather was significantly greater than that of cloudy days. Monthly averaged air temperature and water temperature of each layer increased from March to July, especially in April. Water temperature decreasing with increasing depth was significantly obvious under clear weather than that under cloudy days. The monthly averaged air temperature and water temperature of each layer decreased from August to February of the next year, especially in January. The variation of water temperature with depth took on fall- rise- fall or fall- rise modes. On the seasonal scale, the decreasing rate of water temperature with increasing depth decreased from spring to autumn. Water temperature with increasing depth presented a fall-rise-fall mode in winter. The results could provide guidance for the scientific management of fish ponds in Wuhan, and could also lay foundation of water temperature forecast for aquaculture.