Welcome to Chinese Agricultural Science Bulletin,

Chinese Agricultural Science Bulletin ›› 2015, Vol. 31 ›› Issue (12): 205-209.doi: 10.11924/j.issn.1000-6850.2014-2456

Special Issue: 油料作物

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Optimization of SSR-PCR Reaction System and Primer Screening in Oil Sunflower

Fang Dongmei, Lv Pin, Hou Jianhua   

  1. (College of Agronomy, Inner Mongolia Agricultural University, Huhhot 010019)
  • Received:2014-09-10 Revised:2014-09-10 Accepted:2014-11-05 Online:2015-05-06 Published:2015-05-06

Abstract: In order to obtain the SSR-PCR reaction system for oil sunflower, the method of control variable had been used to optimize the main factors influencing the SSR reaction system, which include the concentration of Mg2 , dNTPs, Taq enzyme, primer, and annealing temperature. Results showed that the optimized SSR reaction system was Mg2 2.5 mmol/L, dNTPs 0.2 mmol/L, Taq polymerase 0.5 U, primer 0.4 μmol/L and the annealing temperature 53.9℃. Through the testing system after optimization, 19 pairs of polymorphic good primer could be used for the next research from 50 pairs of primers screening.