Abstract: To get high-quality in vitro and large-scale production, adopt the affects of cold cathode fluorescent lamp (CCFL) as light emitting materials, the author used bright leaves of Spathiphyllum as the experiment plant material to explore the effectiveness of the cold cathode fluorescent lamp in the production for Spathiphyllum jamesonii in vitro, discussed the best light quality for Spathiphyllum in vitro large-scale production, discussed the perspective of novel radiation resource CCFL in Spathiphyllum in vitro production. The results showed that: the affects of cold cathode fluorescent lamp (CCFL) as light source on Spathiphyllum in vitro culture: the stem length, leaf blade, blade width, roots and root length all reached the maximum in the white CCFL light, and the fresh weight also reached the maximum, followed by the CCFL of the red/blue ratio of 7:3, and they were better than the CK. The dry matter rate and root vigour reached the maximum in the CK, but the soluble sugar content reached the maximum in CCFL of the red/blue ratio of 7:3. The Spathiphyllum in vitro grew best in CCFL of the red/blue ratio of 7:3. White light consisted of plantlets and 70%R 30%B treatment under the best grown status, and was better than the control treatment.