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Chinese Agricultural Science Bulletin ›› 2015, Vol. 31 ›› Issue (17): 22-27.doi: 10.11924/j.issn.1000-6850.casb15020023

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Preparation and Identification of the Artificial Antigen of Ivermectin

  

  • Received:2015-02-03 Revised:2015-05-15 Accepted:2015-03-26 Online:2015-07-27 Published:2015-07-27

Abstract: The purpose of this experiment was to prepare complete artificial antigens of ivermectin. The synthesis of ivermectin half succinate which had hapten structure was performed through introducing active carboxyl group. Artificial antigens, named IVM- BSA and IVM- OVA, were obtained by means of coupling ivermectin with macromolecular carriers, such as bovine serum albumin (BSA) and ovalbumin (OVA), using NHS method. The identification of artificial antigen was conducted by SDS-PAGE and UV scanning methods subsequently. Balb/c mice were immunized with complete antigen IVM-BSA and antiserum titer was tested by indirect competitive ELISA method. The results showed that the coupling ratio of IVM-BSA and IVM-OVA were 14.1:1 and 14.7:1 respectively. The antiserum titer was 1:12800. It came to the conclusion that IVM-BSA and IVM-OVA were synthesized successfully and had satisfied immunogenicity.