Abstract: TUB of Cymbidium faberi was cloned and analyzed in sequence, and the results would provide an important basis for selecting a reference gene and studying the function of TUB. According to the conserved sequences of the TUB homologous genes in GenBank, a pair of primers was designed. TUB genes were identified from the pedicels of C. faberi by homologous cloning. Five TUB genes were successfully cloned and characterized. The sequencing result showed that each of the five TUB gene fragments contained 1055 bp， encoding a protein of 351 amino acids. Each TUB contained the signature sequence Tubulin- beta mRNA autoregulation signal (MREI) and Tubulin subunits alpha, beta, and gamma signature (GGGTGSG). They shared 91.72% nucleotide sequence homology with each other and 85% nucleotide sequence homology with Triticum aestivum. The deduced amino acid sequence shared 97% homology with TUB of Populus trichocarpa, Ricinus communis, Vitis vinifera. The cloned genes were TUB gene fragments, named as CfTUB1, CfTUB2, CfTUB3, CfTUB4 and CfTUB5, respectively. They were registered into GenBank(respective accession number: JN177713, JN177714, JN177715, JN177716 and JN177717).