Effect of Immunostimulants on Intestinal Microorganisms of Sea Cucumber, Apostichopus japonicus

doi:10.11924/j.issn.1000-6850.2014-1753

Abstract

Abstract: In order to study interaction and effect between intestinal microflora and immunostimulants, it was conducted to determine their character and metabolism on intestinal microorganisms of Apostichopus japonicus through Codonopsis. Bacterium was isolated from the intestinal of healthy A. japonicus fed with codonopsis, and identified by 16S DNA. Cross-streak method and paper disk method were used in the antagonism experiment against Vibrio splendidus, and biochemical characteristics were conducted. The results showed that 18 bacteria were from different genera, they were Pseudoalteromonas sp, Bacillus sp, Shewanella sp, Acinetobacter sp, Vibrio sp. The inhibitory test showed that the inhibitory mechanism of 4 superiority strains were competing for living space and producing antibacterial substance. They had different substrate specificity, their protease and amylase activities were high. The oxidase, sucrose and lysine tests showed different biochemical characters. Codonopsis can control and optimize the intestinal function of A. japonicus, improve metabolism and their immunity.

References

[1] 张琴,麦康森,张文兵,等.饲料中添加硒酵母和维生素 E 对刺参生长、免疫力及抗病力的影响[J].动物营养学报,2011,23(10):1745- 1755.
[2] 李继业.养殖刺参免疫学特征与病害研究[M].青岛:中国海洋大学,2008.
[3] 樊英,王淑娴,李天保,等.微胶囊剂型黄芪多糖对刺参生长性能、免疫力及抗病力的影响[J].渔业科学进展,2013,34(1):119-125.
[4] 陈效儒,张文兵,麦康森,等.饲料中添加甘草酸对刺参生长、免疫及抗病力的影响[J].水生生物学报,2010,34(4):731-738.
[5] 樊英,王淑娴,叶海斌,等.黄芪多糖对仿刺参非特异性免疫功能的影响[J].水产科学,2010,29(6):321-324.
[6] 晏永新,张丽,贾海芳,等.党参多糖口服液对小鼠免疫功能的影响[J].中国兽药杂志,2013,47(3):18-20.
[7] 杨希山,编译.肠道免疫学[J].国外医学消化索疾病分册,1995,15 (1):25-27.
[8] 韩立丽,王建发,王凤龙,等.黄芪多糖对肠道免疫功能影响的研究进展[J].中国畜牧兽医,2009,36(8):133-135.
[9] 黄玉章,林旋,王全溪,等.黄芪多糖对罗非鱼肠绒毛形态结构及肠道免疫细胞的影响[J].动物营养学报,2010,22(1):108-116.
[10] 汪婷婷,孙永欣,徐永平,等.多糖类免疫增强剂对海参肠道菌群的影响[J].饲料工业,2008,29(4):19-20.
[11] Chen J X.Present status and prospects of sea cucumber industry in China[A].In: Advances in sea cucumber aquaculture and management.(Eds.by LovatelliA, Conand C, Purcell S, Uthicke S, Hamel J F & Mercier A), FAO,Rome,2004:25-38.
[12] 李成林,宋爱环,胡炜,等.山东省刺参养殖产业现状分析与可持续发展对策[J].渔业科技进展,2010,31(4):26-33.
[13] 隋锡林,刘学光,王军.辽宁省刺参养殖现状及对若干关键问题的思考[J].水产科学,2010,29(11):688-690.
[14] Wang Y G, Zhang C Y, Rong X J,et al.Diseases of cultured sea cucumber (Apostichopus japonicus) in China.In: Advances in sea cucumber Aquaculture and management.Lovatelli A, Conand C, Purcell S, Uthicke S, Hamel J F & Mercier A, (Eds.), FAO, Rome,2004: 297-310.
[15] Zhang Q, Mai K S, Zhang W B, et al.Effects of Dietary Selenoyeast and Vitamin E on Growth, Immunity and Disease Resistance of Sea Cucumbers (Apostichopus japonicus Selenka)[J].Chinese Journal of Animal Nutrition,2011,23(10):1745-1755.
[16] Zhao Y C, Ma H M, Zhang W B, et al.Effects of dietary β-glucan on the growth, immune responses and resistance of sea cucumber, Apostichopus japonicus against Vibrio splendidus infection[J].Aquaculture,2011(315):269-274.
[17] 樊英,王淑娴,李天保,等.微胶囊剂型黄芪多糖对刺参生长性能、免疫力及抗病力的影响[J].水产科学,2013,34(1):119-125.
[18] 王淑娴,李天保,樊英,等.茯苓多糖对刺参体腔液中免疫因子活性的影响[J].饲料研究,2010(1):59-61.
[19] 常杰.对虾和刺参敏感免疫学指标的筛选和评价[D].青岛:中国海洋大学,2010.
[20] 周慧慧,马洪明,张文兵,等.仿刺参肠道潜在益生菌对稚参生长、免疫及抗病力的影响[J].水产学报,2010,34(6):955-963.
[21] 李淮德.应用微生物分子生态学方法研究对虾肠道细菌组成及其变化规律[D].青岛:中国科学院海洋研究所,2010.
[22] 尾崎久雄.李爱杰,沈宗武译.鱼类消化生理[M].上海:科技出版社, 1985.
[23] Korakli M, G?nzle M G, Vogel R F.Metabolism by bifidobacteria and lactic acid bacteria of polysaccharides from wheat and rye, and exopolysaccharides produced by Lactobacillus sanfranciscensis[J].Journal of Applied Microbiology,2002,92(5):958-965.
[24] Helena Tlaskalová- Hogenová, Renata ?těpánková, Tomá? Hudcovic.Commensal bacteria (normal microflora), mucosal immunity and chronic inflammatory and autoimmune diseases[J].Immunology Letters,2004(93):97-108.
[25] 杨世平,吴灶和,简纪常.饲料中添加沼泽生红冬孢酵母对凡纳滨对虾消化酶及肠道微生物的影响[J].水产科学,2011,30(7):391- 394.
[26] 宫魁,王宝杰,刘梅,等.全营养破壁酵母对仿刺参非特异性免疫及肠道菌群的影响[J].中国水产科学,2012,19(4):641-646. [27] 李虹宇,张公亮,侯红漫,等.仿刺参相关微生物对致病灿烂弧菌的拮抗及机理研究[J].食品工业,2012,33(9):117-120.