Effect of X-ray on Inactivating Sperms by Radiating Intravital Macrobrachium nipponensis

doi:10.11924/j.issn.1000-6850.casb15010228

Chinese Agricultural Science Bulletin ›› 2015, Vol. 31 ›› Issue (20): 35-38.doi: 10.11924/j.issn.1000-6850.casb15010228

Effect of X-ray on Inactivating Sperms by Radiating Intravital Macrobrachium nipponensis

Yan Yuedi¹, Fu Hongtuo^1,2, Liang Guoxia³, Qiao Hui², Bai Hongkun³, Jiang Sufei², Xiong Yiwei², Gong Yongsheng², Jin Shubo², Zhang Wenyi², Lv Yang³, Wu Yan²

(¹College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 200000;²Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences/Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Wuxi Jiangsu 214081;³Wuxi Fishery College, Nanjing Agricultural University, Wuxi Jiangsu 214081)

Received:2015-01-29 Revised:2015-06-29 Accepted:2015-04-24 Online:2015-07-28 Published:2015-07-28

Abstract

Abstract: To investigate the effect of X-ray on sperm inactivation of Macrobrachium nipponense, male shrimps were irradiated by 100 kV-5 mA X-ray for 0, 30, 60, 90, 120 min with a distance of 10 cm. Then the male shrimps were made to mate with female soft-shelled shrimps. Broody days and aberration rate were recorded after ovulation. The results showed that there was no difference between 0 min group and 30 min group, and the aberration rate was 0. Broody days gradually decreased while aberration rate increased with increasing irradiation time from 30 to 90 min. After 90 min, broody days showed no change, and the aberration rate reached a peak of 100%. In this research, the radiation time range was 0-120 min. According to the results, the optimum irradiation time to inactivate genetic material of sperms of Macrobrachium nipponense was 90- 120 min.

Yan Yuedi,Fu Hongtuo,Liang Guoxia,Qiao Hui,Bai Hongkun,Jiang Sufei,Xiong Yiwei,Gong Yongsheng,Jin Shubo,Zhang Wenyi,Lv Yang and Wu Yan. Effect of X-ray on Inactivating Sperms by Radiating Intravital Macrobrachium nipponensis[J]. Chinese Agricultural Science Bulletin, 2015, 31(20): 35-38.

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Effect of X-ray on Inactivating Sperms by Radiating Intravital Macrobrachium nipponensis

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