Changes in IAA and ABA Levels and PpPINs Genes Expression During the Bud Dormancy Induction in Peach

doi:10.11924/j.issn.1000-6850.casb15030080

Abstract

Abstract: This study focused on the changes of physiology and relative gene expression during the bud dormancy induction in peach. The contents of IAA and ABA were measured by HPLC and the relative genes expression of auxin polar transport carrier genes PpPIN1, PpPIN3, PpPIN5 and PpPIN8 was detected by RTqPCR in leaves and vegetative buds. Three peach varieties,‘Zhongnonghongjiubao’,‘Zhongnongsanhao’, 03- 30-035, were chosen as the test materials. Results showed that during the bud dormancy induction in peach, the content of IAA did not show a significant change, but the content of ABA rose since October 6 and then reached the peak on October 16. Nevertheless, the content of IAA surged in leaf buds while the content of ABA decreased gradually. Among the four genes mentioned above, PpPIN5, followed by PpPIN3, was in active expression in leaves and had a strong expression in the early to mid-October. However, the relative expression of PpPIN1 and PpPIN3 dropped suddenly in October 26 in vegetative buds. In general, the changes of endogenous hormones and PpPINs genes relative expression were significantly different in leaves and vegetative buds during the bud dormancy induction in peach.

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