Cloning and Expression Analysis of Glutathione S-transferase Gene SoGST-1a in Sugarcane

doi:10.11924/j.issn.1000-6850.casb15040019

Abstract

Abstract: The glutathione S-transferase (GST) gene from sugarcane was cloned. Its sequence characteristics and expression patterns were analyzed. This provided the source of gene in sugarcane breeding for drought resistance. In this study, a GST gene from sugarcane variety ROC22 was cloned by in silico cloning and RT-PCR, named SoGST-1a. Its sequence characteristics were analyzed by using bioinformatics software, and its expression patterns were detected by using real-time PCR. Sequence analysis showed that the full length cDNA of SoGST-1a was 702 bp, which encoded 224 amino acids. The predicted protein molecular formula was C₁₁₁₇H₁₇₅₀N₃₀₀O₃₂₈S₆, whose molecular mass was 24.82 kDa and isoelectric point was 5.96. Hydrophobic analysis indicated that it was a hydrophilic protein. Conserved domain prediction showed that SoGST-1a protein had?two GST domains. Phylogenetic analysis showed that SoGST-1a protein was close to Zeta class GST protein of maize. Real-time PCR results showed that the SoGST-1a gene was down-regulated expression under drought treatment. The research results showed that SoGST-1a gene might not play a role in drought stress in sugarcane. These results provided a basis for further understanding biological function of SoGST-1a.

Xu Lei,Yao Yanli,Hu Xiaowen,Xing Shulian and Liu Yang. Cloning and Expression Analysis of Glutathione S-transferase Gene SoGST-1a in Sugarcane[J]. Chinese Agricultural Science Bulletin, 2015, 31(26): 71-77.

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