Abstract: The purpose of this paper was to test species composition and occurrence of beetles (Coleoptera) in ryegrass (Lolium perenne) artificial pasture in Dushan County of Guizhou. Sweeping method, pitfall traps along with ocular estimation were used in the field collections, and the occurrence of beetles was summarized based on the collections. 33 species of beetles which belonged to 10 families in Coleoptera were identified. Chaetocnema concinnicollis was the dominant species. The main pests were Atrachya menetriesi, Basilepta fulvipes, Longitarsus succineus, Pagria signata, Neomyllocerus hedini, Nothomyllocerus illitus, Agrypnus musculus and Apogonia cribricollis. The main natural enemy beetles were Chlaenius (Achlaenius) micans, Chlaenius (Pachydinodes) virgulifer, Harpalus sinicus, Pheropsophus javanus, Cylindera kaleea, Brumoides sp. and Propylea japonica. The quantity of other species was low. The species composition of beetles was relatively stable. Chaetocnema concinnicollis was potential beetle pest, which might possibly become one important pests of artificial pasture. The conservation and utilization of natural enemy should be considered in pest control.