Abstract: AlsCBF gene from the Amygdalus ledebouriana Schleche was cloned and the gene related information was analyzed. Thus the study could provide a theoretical basis for the identification of gene function, and provide a source of gene for plant cold-resistance breeding. The full-length of cDNA of plant defense pathway transcription factor was isolated from Amygdalus ledebouriana Schleche by RT-PCR and RACE, which was named AlsCBF and submitted to GenBank (Accession number: HQ908653). According to the bioinformatics analysis, the cloned AlsCBF gene was 1171 bp including code segment 714 bp, encoding 237 amino acids, the predicted AlsCBF protein’s molecular formula was C₁₁₅₆H₁₈₃₂N₃₃₂O₃₅₆S₁₅, whose relative molecular mass was 26.5581 kDa and isoelectric point was 6.76. It was predicted that this protein was hydrophilic protein, and there was no signal peptide. Characteristic prediction results indicated that the secondary structure of AlsCBF protein mainly included α-helix, β-sheet and random coil. And there were eight serine sites, one threonine sites. Three-dimensional structure predicted that AlsCBF protein confirmed the characteristics of AP2 domain model. In this study, related information was obtained by means of bioinformatics analysis of AlsCBF gene, and through comparing with previous studies, the gene played an important role in the plant cold-resistance.