Abstract: In order to explore the embryonic callus conditions and establish a preliminary system of somatic embryogenesis of Eucommia ulmoides, the influence of different hormone proportions in medium and immature embryo age and genotype on the induction of embryogenic callus of Eucommia ulmoides was explored using young leaves, immature zygotic embryos as explants. In addition, embryogenic callus was distinguished via the morphology and cytology. The results showed that the leaf callus induced from 4 kinds of different hormone proportion mediums were different in color and texture, in the medium of MS 2,4-D 2 mg/L 6-BA 1 mg/L and MS 2,4-D 2 mg/L 6-BA 0.5 mg/L, embryogenic callus of leaves was easier to be induced. In the medium of MS 2,4-D 2 mg/L 6-BA 1 mg/L, the callus induced from the immature zygotic embryos could be divided into four categories: the types of spherical and granular callus were embryogenic. The immature zygotic embryos acquisited from different periods had significant differences in callus induction rates and June 14^th showed the highest callus induction rate, while callus induction of different genotypes had no significant difference.