Influence Factors of Inducing Sleeping Buds on Stalk of Phalaenopsis Orchids with Large Red Flowers

doi:10.11924/j.issn.1000-6850.casb15110103

Abstract

Abstract: The paper aims to study the influence of 6-benzylaminopurine (6-BA) on germination of sleeping buds on stalks of moth orchid (Phalaenopsis) varieties, and the influence of sleeping buds with different degrees of lignification on the success rate of the bud germination. In this experiment, three varieties of ‘Big Chili’, ‘Red Swan’ and ‘Alishan Guniang’ were cultured in vitro, to count up the success rate of germination and the growth situation of the stalk sleeping buds. The results indicated that: (1) when the buds were in half lignification, stalk had the hightest success rate of germination, which was up to 100%; (2) in all culture mediums, MS 6-BA 5.0 mg/L was the most suitable one for the buds germination of ‘Red Swan’ and ‘Alishan Guniang', and MS 6-BA 4.0 mg/L was the most suitable one for the buds germination of ‘Big Chili’. The experiment successfully obtained the most appropriate lignification degrees for the sleeping buds germination on stalks of the three varieties, and the most appropriate culture mediums of the sleeping buds germination, therefore, it could provide certain theoretical basis for establishing a production system of tissue culture and rapid reproduction of different varieties of moth orchid with large red flowers.

CLC Number:

S688.9

Tan Jing,Zhang Yingjie,Guo Wenjiao and Liu Xueqing. Influence Factors of Inducing Sleeping Buds on Stalk of Phalaenopsis Orchids with Large Red Flowers[J]. Chinese Agricultural Science Bulletin, 2016, 32(16): 87-92.

References

[1] 李淑英,张颖,王小婧.蝴蝶兰的组织培养[J].农业工程技术·温室园艺,2014,11(11):78.
[2] 苏宁.兰花历史与文化研究[D].北京:中国林业科学研究院,2014.
[3] Christenson E A. Phalaenopsis: A Monograph[M].London: Timber Press,2001:39-291.
[4] 李小康,白天明,王志毅,等.洋兰引种栽培、展示及应用[J].种业导刊,2015,9:23-25.
[5] 肖善军.兰花分株繁殖与盆栽护理技术[J].现代农业科技,2015,9(9):165-166.
[6] 刘海珊,蝴蝶兰组培苗生产成本研究[D].北京:北京林业大学,2008.
[7] 杜君,王利民,孟月娥,等.我国蝴蝶兰产业发展现状及对策研究[J].北方园艺,2015,9(9):172-175.
[8] 袁园,陈和明,吕复兵,等.12个蝴蝶兰品种主要性状的主要主成分分析[J].广东农业科学,2015,42(13):54-59.
[9] 王芳,文峰,武斌,等.激素对蝴蝶兰快速繁殖的影响[J].新疆农业大学学报,2003,26(4):68-71.
[10] Chen J T, Chang W C. Direct Somatic Embryogenesis and Plantregeneration from Leaf Explants of Phalaenopsis amabilis[J]. Biologia Plantarum,2006,50(2):169-173.
[11] 李云.林果花菜组织培养快速育苗技术[M].北京:中国林业出版社,2001:6.
[12] 陈桂敏,郑羽书,杨佩华,等.不同外植体对蝴蝶兰组培快繁的影响[J].中国园艺文摘,2013,11(11):15-16.
[13] 马杰,何蔚荭,崔波.蝴蝶兰原球茎状体的诱导及增殖[J].河南科学,2005,23(1):51-53.
[14] 杨善云.蝴蝶兰组织培养的研究进展[J].园艺与种苗,2012(9):59-61.
[15] 曾碧玉,许传俊,张文惠,等.蝴蝶兰花梗初代培养研究[J].福建农业学报,2013(2):124-128.
[16] 秦凡,周吉源.蝴蝶兰的组织培养研究[J].生物学杂志,2003,20(3):19-21.
[17] 王玲,陈发棣,陈凤,等.蝴蝶兰花梗芽的初代诱导[J].江苏农业科学,2012,40(12):68-71.
[18] 鲁雪华,郭文杰,徐立晖,等.蝴蝶兰花梗节间段培养繁殖的初步研究[J].园艺学报,2002,29(5):491-492.
[19] 高立鹏,邓茜玫.我国年宵花市场现状、问题及对策[J].云南农业大学学报:社会科学版,2014,6(6):64-68.
[20] 靖晶.蝴蝶兰组织培养与脱除病毒技术研究[D].北京:北京林业大学,2011.
[21] 王玲,陈发棣,陈凤,等.不同细胞分裂素及使用浓度对蝴蝶兰花梗芽增殖生长的影响[J].江苏农业科学,2013,41(2):49-51.
[22] 曹修才,杨士辉,朱方平,等.蝴蝶兰组培技术研究与产业化开发[J].北方园艺,2006(6):150-151.
[23] 许聪耀译.控制蝴蝶兰梗芽培养生长的影响因子[J].中国兰艺,1985,8(4):12-16.
[24] 陆顺教,易双双,任羽,等.秋石斛新生侧芽中部茎段组培快繁体系的建立[J].南方农业学报,2015,46(8):85-90.
[25] 吕炯璋,高琼,李智勇.温度处理对蕙兰植株形态建成的影响[J].北京农业职业学院学报,2015,29(127):31-37.
[26] Dalal M A, Sharma B B, Rao M S. Studies on Stock Plant Treatment and Initiation Culture Mode in Control of Oxidative Browning in Vitro Cultures of Grapevine[J]. Scientia Horticulturae,1992,51(1-2):35-41.
[27] 张和.降低蝴蝶兰组培苗褐变率的技术研究[D].杨凌:西北农林科技大学,2014.
[28] 刘昕,王清,余斌.不同外源激素对胀果甘草愈伤组织诱导及褐化的影响[J].甘肃农业大学学报,2010(6):88-93.