Advances in Protoplast Fusion Technology

doi:10.11924/j.issn.1000-6850.casb16010076

Abstract

Abstract: In the present research, advances in protoplast fusion technology were reviewed. Starting from the steps of protoplast fusion that included protoplast isolation, culture and fusion, detailed descriptions about research progress in protoplast isolation, culture and fusion were respectively expounded, and relevant factors affecting the protoplast fusion were analyzed. Then, the method and mode of protoplast fusion as well as the screening and identification of hybrid cell obtained from protoplast fusion were summarized. Based on the analysis of the relevant literature, the methods widely used in screening and identification of hybrid cell were summed up. Finally, the problems of protoplast fusion in breeding practice were analyzed, and prospects of this technology in breeding were also outlined, which could lay a foundation for the development of protoplast fusion technology.

CLC Number:

S336

References

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