Abstract: In order to more fast, easily and precisely detecting sulfonamides residues in aquatic products, dispersive solid phase extraction (dSPE)-ultra performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) was established. Three methods of purification, including dSPE, rotary evaporation condensation and the national standard purified method, were compared in this study. Aquatic samples were detected by UPLC-MS/MS after dilution. The results showed that dSPE was more fast, accurate and simple in dealing with aquatic samples than the other two methods. The calibration curves for selected sulfonamides (concentration range of 0.5~50 μg/L) were linear with correlation coefficients more than 0.9. Detection limits of instrument and method were 0.0007~0.002 μg/L and 0.007~0.02 μg/kg respectively. And limits of quantitation ranged from 0.02 μg/kg to 0.08 μg/kg. The recovery and RSD were 67.0%~173.5% and 2%~24.5% respectively. In a word, this method is fast, simple and accurate, can be developed for detecting and analyzing sulfonamides residues, and also provides a reference for research of fast and accurately detecting sulfonamides residues.