Abstract: To study the tissue culture and rapid propagation technique of Liriope platyphylla, we analyzed the effects of plant growth regulator on callus induction, bud differentiation and test- tube plantlet rooting of rhizome bud and leaf. The results showed that MS+BA 1.0 mg/L+NAA 1.0 mg/L was the best medium to induce the callus of rhizome bud, and the induction rate was 100%; MS+BA 1.0 mg/L+NAA 0.5 mg/L was the ideal medium to induce the callus of leaf, and the induction rate was 52%. The induction rate and bud multiplication of rhizome bud callus were significantly higher than that of leaf callus, the highest induction rate was 100%, and the highest propagation times was 4.6. 1/2MS+IBA 0.5 mg/L and 1/2MS+IAA 0.25 mg/L medium were suitable for test-tube plantlet rooting, the rooting rate was 100% and the average rooting number was above 5. The survival rate of test-tube plantlet was more than 90% after transplanting.