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Chinese Agricultural Science Bulletin ›› 2017, Vol. 33 ›› Issue (32): 121-126.doi: 10.11924/j.issn.1000-6850.casb16110005

Special Issue: 生物技术 水产渔业

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Population Genetic Structure of Acrossocheilus wenchowensis in Mountainous Area of Southern Anhui Province Based on Mitochondrial Cyt b gene

  

  • Received:2016-11-01 Revised:2017-11-07 Accepted:2016-12-15 Online:2017-11-27 Published:2017-11-27

Abstract: In order to study the population genetic structure of Acrossocheilus wenchowensis in mountainous area of southern Anhui Province, mitochondrial cytochromeb (Cytb ) gene from 5 populations (Qimen, Yixian, Xiuning, Jingde and Ningguo) with 131 individuals of A. wenchowensis were amplified and sequenced bidirectionally. Finally 14 haplotypes were identified and 38 mutation sites were detected. The average contents of T, C, A and G were 27.0%, 29.7%, 28.2% and 15.1% respectively, A+T content (55.2%) was higher than G+ C content (44.8%). The haplotype diversity (0.0000-0.6799) and nucleotide diversity (0.0000-0.00759) of the 5 populations were all low. The genetic differentiation index (F ST) of populations ranged from 0.2916 to 0.9782. Analysis of molecular variance (AMOVA) indicated that there was 52.74% of variance among populations, suggesting that populations had significant genetic differentiation. But there was no obvious genetic difference among populations from different rivers. The molecular phylogenetic tree (NJ- tree) showed that the 5 populations could be divided into two groups, with Qimen and Jingde populations clustered into group 1 and the other three populations clustered into group 2. The results were related to geographic isolation and ecological habit of A. wenchowensis.

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