Colonizing Dynamics of Endophytic Diazoteophic Bacteria DENB20 in Different Desert Plant Species

doi:10.11924/j.issn.1000-6850.casb16040072

Abstract

Abstract: Endophytic diazoteophic bacteria DENB20 belonged to Klebsiella Trevisan. In order to investigate the colonizing dynamics of DENB20 in different desert plant species, this strain was marked by Rif and Str and was studied its colonizing dynamics in different desert plant species. The results showed that the population of DENB20 grew continuously in the roots of Tamarix ramosissima after inoculation, but in the stems and leaves of Tamarix ramosissima, the population of DENB20 decreased gradually and could not be isolated after 50 d inoculation. Although the population decreased gradually in the roots, stems and leaves of Lycium ruthenicum, there was still a certain amount of strain after 80 d inoculation. The development trend of DENB20 in the root of Poacynum hendersonii was first decreasing then increasing, but the population of DENB20 decreased gradually in the stems and leaves and could not be isolated after 50 d inoculation. In conclution, endophytic diazoteophic bacteria DENB20 could colonize stably in the roots of Tamarix ramosissima, Lycium ruthenicum and Poacynum hendersonii, and the colonization ability of DENB 20 in roots was as follows: Poacynum hendersonii＞Tamarix ramosissima＞Lycium ruthenicum.

CLC Number:

S154.3

References

[1]张军民. 新疆干旱区绿洲特征分析及生态保护对策研究[J].生态环境,2007,16(4):1328-1332.
[2]钱亦兵,樊自立,雷加强,等.近五十年新疆水土开发及引发的生态环境问题[J].干旱区资源与环境,2006,20(3):58-63.
[3]韩桂红,吐尔逊.哈斯木,石丽.塔里木河下游土地沙漠化及其原因探讨[J].中国沙漠,2008,28(2):217-222.
[4]张丽梅,方萍,朱日清.禾本科植物联合固氮研究及其应用现状展望[J].应用生态学报,2004 ,15 (9):1650-1654
[5]Pan B,Vesse J K.Response of gluconacetobacter diazotrophicus on solid media to change in atmospheric partial O2 pressure[J].Applied and Enviromental Microbiology,2001,67(10):4694-4700.
[6]Martinez-Viveros O,Jorquera M A,Crowley D E,et al.Mechanisms and practical considerations involved in plant growth promotion by rhizobacteria[J].Journal of Plant Nutrition and Soil Science, 2010,10(3):293–319.
[7]Bin Y,Saito A,Minamisawa K,et al.Effect of inoculation with anaerobic nitrogen-fixing consortium on salt tolerance of Miscanthus sinensis[J].Soil Science and Plant Nutrition,2005,51(2):243-249.
[8]殷水宁,孙广宇.内生细菌YSN-7提高小麦耐盐性研究[J].西北农林学报,2007,16(5):282-284.
[9]Ali B, Sabri A N, Hasnain S. Rhizobacterial potential to alter auxin content and gowth of Vigna radiata (L.). World Journal of Microbiology and Biotechnology ,2010,26(8):1379–1384.
[10]Bhattacharyya P N, Jha D K. Plant growth promoting rhizobacteria (PGPR): emergence in agriculture[J]. World Journal of Microbiology and Biotechnology, 2012,28:1327–50.
[11]Gururani M A,Upadhyaya C P,Baskar V,et al.Plant growth-promoting rhizobacteria enhance abiotic stress tolerance in Solanumtuberosum through inducing changes in the expression of ROS-scavengingenzymes and improved photosynthetic performance[J].Journal of Plant Growth Regulation ,2013,32(2):245-258.
[12]Sang H J,Mayank A G,Se-Chul C.Isolation and characterization of plant growth promoting endophytic diazotrophic bacteria from Korean rice cultivars[J].Microbiological Research,2014,169 (1):83–98
[13]Duangpaeng A, Phetcharat P, Chanthapho S,et al.The Study and Development of Endophytic Bacteria for Enhancing Organic Rice Growth[J].Procedia Engineering ,2012,32:172–176.
[14]徐正金,罗明,王纯利.3种典型荒漠灌木内生固氮菌及固氮酶基因nifH多样性分析[J].中国沙漠,2014,34(2):472-480.
[15]季华,潘存德,周俊.荒漠灌木内生固氮菌对环境因子的适应性研究[J].中国沙漠,2011,31(4):942-947.
[16]王卫霞.新疆几种典型荒漠植物根际微生物特征及内生固氮菌的分离、促生性能研究[D].乌鲁木齐: 新疆农业大学硕士学位论文,2009:21－33.
[17]Krause A,Ramakumar A,Bartels D, et al.Complete genome of the mutualistic,N2-fixing grass endophyte Azoarcus sp.strain BH72[J].Nature Biotechnology,2006,24(11):1385–1391.
[18]Amandeep S B.Insolation of endophytic bacteria from Lodgepole pine and Western red cedar and determination of their nitrogen fixing ability(M.Sc.Thesis) [D].Canada: The university of British Columbia,2003.
[19]罗明,芦云,张祥林,等.内生拮抗细菌在哈密瓜植株体内的传导定殖和促生作用研究[J].西北植物学报,2007,27(4):720-725.
[20]芦云.新疆哈密瓜内生细菌的种群、分布动态及其抗菌作用研究[D].乌鲁木齐:新疆农业大学, 2005:36-37.
[21]Triplett E W,Elkan G H,Upchurch R G. Diazotrophic endophytes:progress and prospects for nitrogen fixation in monocots[J].Plant and soil, 1996,9:29-38.
[22]何平平.水稻内生固氮细菌生长条件、发酵营养及在植株体内外定殖研究[D].扬州:扬州大学,2007:32-35.
[23]路国兵,张瑶,翼宪领,等.植物内生细菌的侵染定殖规律研究进展[J].生物技术通报,2007, 18(3):88-92.
[24]Gyaneshwar P,James E K,Mathan N,et al.Endophytic colonization of rice by a diazotrophic strain of Serratia marcescens [J].Journal of Bacteriology,2001,183(8):2634－2645.
[25]陶艳会,林会,赵斌.内生固氮菌HAUM10对水稻的侵染定殖规律及促生效应[J].湖北农业科学,2012,51(6):1257-1262
[26]Compant S,Mitter B,Colli-Mull J G,et al. Endophytes of grapevine flowers, berries, and seeds: identification of cultivable bacteria, comparison with other plant parts, and visualization of niches of colonization[J].Microbial Ecology,2011, 62(1):188-197.
[27]Tomblini R,Unge A,Davey M E,et al.Flow cytometric and microscopic analysis of GFP-tagged pseudomonas fluorescens bacteria[J]. FEMS Microbiology Ecology, 1997,22:17-28.