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Chinese Agricultural Science Bulletin ›› 2019, Vol. 35 ›› Issue (14): 47-53.doi: 10.11924/j.issn.1000-6850.casb18060068

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Optimization of Apical Meristem in Vitro Culture Condition of‘1st Ning Waxy Taro’

  

  • Received:2018-06-14 Revised:2018-08-11 Accepted:2018-08-24 Online:2019-05-15 Published:2019-05-15

Abstract: To enrich the taro germplasms in Fujian Province and accelerate the application of elite varieties, using the apical bud and lateral bud of‘1st Ning waxy taro’tuber as explants, adventitious buds were induced from apical meristem culture. Based on the orthogonal experiment, the two factors of TDZ concentration and temperature were optimized using adventitious bud multiplication coefficient as a response value with central composite design of response surface method. The results showed that TDZ was more appropriate for the clustered buds induction from apical meristem, compared with 6-BA. The bud germination was significantly promoted on MS medium when supplemented with 0.1-0.2 mg/L TDZ, and the planting rates were 76.7%- 80.0%. ELISA of taro mosaic virus (DMV) detection kits showed that the detoxification rate of induced bud was 90%. The response surface method showed that the optimal culture condition of adventitious buds subculture multiplication was MS +TDZ 0.31 mg /L, and the culture temperature was 30.32℃. The predicted value of multiplication coefficient was 9.08, while the actual value was 8.86. Therefore, the response surface method can effectively optimize the subculture multiplication condition of adventitious buds from‘1st Ning waxy taro’ .