Abstract: It aims to provide a basis for cultivation of Paris polyphylla var. yunnanensis and application of LED light source. Experimental light source was the elemental blue LED light with wavelength of 460 nm, 3-yearold seedlings of P. polyphylla var. yunnannens were cultivated under the light intensity treatment of 50, 100, 150, 200 μmol/(m2 ·s) respectively to observe their growth status. The results showed that the plant height, ground biological yield, leaf net photosynthetic rate, and underground biological yield of P. polyphylla var. yunnanensis were the highest at 150 μmol/(m2 ·s) treatment, the stomatal conductance and transpiration rate were the highest under the treatment of 100 μmol/(m2 ·s) and the lowest at 200 μmol/(m2 ·s) treatment. The effects of different light intensities on light energy conversion efficiency and reaction activity of P. polyphylla var. yunnanensis were not significant. The contents of different saponin components and total saponin in rhizome increased and then decreased with the increase of blue light intensity. The saponin content was the highest at 100 μmol/(m2·s) treatment and the lowest at 200 μmol/(m2·s) treatment. Comprehensive analysis of the plant growth condition, leaf photosynthetic characteristics and saponin content indicates that the blue light intensity of 150 μmol/ (m2·s) is the most favorable topromote the leaf growth and photo synthesisand 100 μmol/(m2·s) is the most favorable light intensity for the accumulation of rhizome saponin in P. polyphylla var. yunnanensis. It is suggested that the blue light intensity should be controlled at 100- 150 μmol/(m2 · s) in the facility cultivation of P. polyphylla var. yunnannensis.