Abstract: An ion chromatographic method for the determination of potassium, sodium, calcium, magnesium and ammonia in tobacco was developed. The potassium, sodium, calcium, magnesium and ammonia were extracted from the samples by ultrasonic extraction with 5% hydrochloric acid solution in an extraction bottle with solid phase extraction and purification function, and the extract were purified by solid phase extraction with graphitized carbon black. The purified samples were separated on an IonPacCS12A (3?150 mm，5 μm) cation ion exchange column with 30 mM methanesulfonic acid as mobile phase. The five cation ion (K+、Na+、Mg2+、Ca2+、and NH4+) were separated within 5.0 min. The sample extraction, purification, and filter can be completeed in our self-designed extraction bottle without any sample transfer. Compared with the conventional method, the operation process is simplified and the efficiency is greatly improved in new method. In addition, the new method has high sensitivity and high recovery, and provides a high-throughput analysis method for the rapid determination of potassium, sodium, calcium, magnesium and ammonia in gene editing materials.