The Anesthetic Effect of MS-222 on Juvenile Barbuscapito

doi:10.11924/j.issn.1000-6850.casb18040020

Abstract

Abstract: This study aims to investigate the anesthetic effect of MS- 222(Tric-ainemethanesulphonate) on juvenile Barbuscapito. At the temperature of (26±0.5)℃, the anesthetic effects of different concentrations of MS- 222 on juvenile Barbuscapito are observed, including behavior changes during anesthesia and resuscitation, and changes of respiratory frequency during anesthesia. At the same time, the effects of different air exposure times on recovery time and recovery rate of anesthetized juveniles are observed. The test results showed that the anesthetic process of juveniles of Barbuscapito was divided into 6 stages and the recovery process was divided into 4 stages. The effective anesthetic concentration of MS-222 for juvenile Barbuscapito was 120-160 mg/L. With the increase of anesthetic time and concentration, the respiratory rate of juvenile Barbuscapito showed a decreasing trend overall. For juvenile fish entering the 5th anesthetizing stage, when the air exposure time was within 4 min, the recovery time decreased with the increase of the air exposure time; when the exposure time was more than 6 min, the recovery time increased with the exposure time; none of the experiment fish died when exposed within 10 minutes. Experiments prove that MS-222 has good anesthetic effect on juvenile Barbuscapito.

References

[1] 尼科里斯基 L B.分门鱼类学[M].北京：高等教育出版社,1958.186-189.
[2] 蔺玉华,耿龙武,王信海,等.池塘驯养大鳞鲃某些生物学特性研究[J]. 天津师范大学学报：自然科学版,2009,29(2)：72-75．
[3] 徐伟,耿龙武,李池陶,等.北方寒地池养大鳞鲃生长和越冬成活[J]. 淡水渔业,2012,42(1)：68-71
[4] 耿龙武,徐伟,李池陶,等.盐碱对大鳞鲃血清渗透压、离子含量及鳃丝 Na＋/K＋-ATP 酶活力的影响[J].中国水产科学,2011,18(2)：458-465.
[5] 耿龙武,徐伟,李池陶,等.大鳞鲃耗氧率和窒息点的测定[J].上海水产大学学报,2012,21(3)：363-367.
[6] 耿龙武,徐伟,金国雄,等.4种常用水产药物对大鳞鲃的急性毒性试验[J].江西农业大学学报,2011,33(3)：568-572.
[7] 蔺玉华,王信海,耿龙武,等.大鳞鲃(Barbus capito)的含肉率及肌肉营养成分分析[J]. 天津师范大学学报：自然科学版,2008,28(3)：1-4．
[8] 葛彦龙,徐伟,耿龙武. 大鳞鲃鱼同工酶的研究[J]. 水产学杂志,2011,24(4)：59-61.
[9] 鲁翠云,耿龙武,李超,等.微卫星标记分析大鳞鲃养殖群体的遗传结构及生长性状[J].水产学报,2013,37(8):1121-1128.
[10] 耿龙武,徐伟,蔺玉华,等.大鳞鲃人工繁育技术初报[J].吉林农业大学学报,2010,32(2)：218-220.
[11] 徐伟,耿龙武,李池陶,等.大鳞鲃的人工繁殖、胚胎发育和耐盐碱测定[J].水产学报,2011,35(2)：255-260.
[12] 丁辰龙,蔺玉华,王宣朋,等.大鳞鲃的人工繁殖及体色变异分析[J].天津师范大学学报(自然科学版),2016,36(5)：60-64.
[13] MATTSON N S,ＲIPLE T H．Metomidate,a better anesthetic for cod ( Gadus morhua) in comparison with benzocaine,MS-222,chlorobutanol,and phenoxyethanol[J]．Aquaculture,1989,83(1/2) : 89-94.
[14] 王昊龙,徐弘,安苗, 等. MS－222对杂交鲟幼鱼的麻醉效果及模拟运输试验[J].山地农业生物学报,2016,35( 1) : 0040-0044.
[15] 官曙光, 关健, 刘洪军,等. MS-222 麻醉许氏平鲉幼鱼的初步研究[J]. 海洋科学,2011,35(5)：100-105.
[16] 庄平,徐滨,章龙珍等.SMS-222和丁香酚对中华鲟幼鱼耗氧率与排氨率的影响[J].中国水产科学,2009,16(4):612-618.
[17] 王秀华,张烨伟,杨春志. MS-222对牙鲆麻醉效果的研究[J].渔业科学进展,2009, 30(3): 1-6.
[18] 刘长琳,陈四清,何S力等.SMS-222S对半滑舌鳎成鱼的麻醉效果研究[J].中国水产科学,2008,15(1)：92-99.
[19] 王利娟,程守坤,张饮江等.SMS-222S在加州鲈鱼模拟运输中的麻醉效果[J].上海海洋大学学报,2015,24(2):235-241.
[20] MAＲKING L L,MEYEＲ F P．Are batter anesthetics needed in fisheries[J]．Fisheries,1985,10( 6) : 2－5．
[21] 郭丰红,汪之和．MS－222 对鳜鱼成鱼麻醉效果的研究[J].湖南农业科学,2009,7( 12) : 150-153.
[22] 彭宁东,汤文圣,郭栋,等.MS-222 对赤眼鳟幼鱼麻醉效果的研究[J].华南师范大学学报(自然科学版), 2016,48( 6) : 37-43.
[23] 何小燕,袁显春,潘志,等.MS-222 对布氏鲷幼鱼的麻醉效果研究[J]. 四川动物,2013,32(5)：729-733.
[24] COOK S J,SUSKI C D,OSTＲAND K G． Behavioral andphysiological assessment of low concentration of clove oilanaesthetic for handing and transporting largemouth bass(Micropterus salmoides)[J]．Aquaculture,2004,239: 509-529．
[25] 张朝晖,丛娇日,王波,等．麻醉剂丁香酚对黄腊鯵耗氧的影响[J].海洋科学2003,(6) : 11-14; 34．
[26] 杜浩．美洲鲥(Alosa sapidissima)人工孵化、养殖及转运关键技术的研究[D]．武汉: 华中农业大学,2005．
[27]史东杰,梁拥军,孙砚胜,等.MS-222对吉富罗非鱼麻醉效果的初步研究[J].科学养鱼,2015,5:52-53.
[28] 管敏,胡美洪,刘勇,等．MS-222 对长鳍吻鮈幼鱼麻醉效果的研究[J].水产科技情报,2015(2) : 72－77．