Abstract: To provide references for rational irrigation on soybean at later growth stage, eight spring soybean varieties were used as materials in the experiment from 2016 to 2017. After harvesting, protein and fat content were measured by Foss 1241, and the yield was calculated to explore the effect of drought at grain filling stage on quality and yield of spring soybean. The results showed that under drought condition at grain filling stage, the protein content of‘Heinong 37’decreased most obviously, while that of‘Hefeng 46’changed least; the fat content of‘Heinong 61’had the most obvious increase, and the fat content of‘Suinong 29’had the least increase; 100- grain weight of‘Heinong 65’had the most obvious decrease and that of‘Heinong 45’ decreased the least;‘Heinong 65’had the greatest reduction in yield, while‘Heinong 44’,‘Heinong 37’and ‘Heinong 45’had the smallest decrease in yield. The experiment indicats that drought at grain filling stage could decrease protein content and 100-grain weight, increase fat content, and decrease yield obviously.