Abstract: The paper aims to breed new varieties of sun-cured tobacco with high quality, disease resistance and high yield. We introduced 20 germplasm resources of sun-cured tobacco in Heilongjiang, and through the comparison and identification of their genetic diversity and disease resistance, we selected the suitable parent material for the local area. The results showed that: 7 germplasm resources were screened as the parent materials for breeding new varieties, ncluding‘Qinggeng’, ‘Qishishaiyan’, ‘Huangyan-1’, ‘Huangyan-2’, ‘Gaochanyan- 1’,‘Baogaiyan- 1’,‘Nanxiongyan- 2’. 10 different quantitative traits were compared and analyzed, the Shannon- weaver index was between 4.1- 4.3, showing that the phenotypic traits of the 20 germplasm resources had great genetic diversity. The excellent germplasm resources can enrich the local breeding materials, it is conducive to expand the genetic background and improve the breeding efficiency.