Abstract: To understand the production and utilization value of newly developed wheat varieties in the middle and lower reaches of the Yangtze River, the high and stable yield ability of thirteen wheat varieties in the regional trail of the middle and lower reaches of the Yangtze River during 2016-2017 were analyzed with the methods of Francis- Kannenberg genotype- group technique, Eberhart- Russell linear regression model, adaptability parameter and high stability coefficient. The results showed that there were eight varieties (accounting for 61.5%) with a yield increase of more than 2% compared with the control variety‘Yangmai 20’, but there were fewer varieties with high and stable yield, only‘Weihong 225’and‘Yangfumai 2049’, accounting for 15.4%. The results of Francis-Kannenberg genotype-group technique, linear regression model and adaptability parameter for the evaluation of stability of the wheat varieties were basically the same. The correlation analysis showed that high stability coefficient (HSC) had extremely and significantly positive correlation with the average yield, and significantly negative correlation or extremely and significantly negative correlation with standard deviation, coefficient of variation, regression coefficient and adaptability parameter.