Abstract: Amino acids, the basic components of protein, are important nutrients to human. Colored wheat is regarded as a new wheat type for nutrition and health care, but the amino acid contents of colored wheat are still not well known. In this study, we developed three near isogenic wheat lines with blue‘( Kexing 611’), purple ‘( Kexing 617’) or white‘( Jimai 22’) grains using‘Jimai 22’as a recurrent parent. Their kernels harvestedfrom three environments are comparatively analyzed for total amino acid contents with high performance liquid chromatography. The results showed that the contents of essential, non- essential and total amino acids of ‘Kexing 611’were significantly higher than that of‘Kexing 617’and‘Jimai 22’. However,‘Kexing 617’ exhibited lower contents of essential, non-essential and total amino acids compared with those of‘Jimai 22’. Hence, the blue-grained wheat line is a better source of amino acid nutrition for human consumption, which could be used to enhance the amino acid contents of wheat cultivars and thus the nutritional quality of wheat.