Chlorophyll Fluorescence Kinetic Parameters of Saccharum robustum 57NG208 F2 Hybrids Under Water Stress

doi:10.11924/j.issn.1000-6850.casb18070058

Abstract

Abstract: In order to study the effects of chlorophyll fluorescence dynamic parameters on Saccharum robustum 57NG208 F2 materials under different water stress, Used four species Saccharum robustum of 57NG208 and Nanjian sugarcane reciprocal cross offspring, greenhouse design barrels of plant drought resistance in drought resistance test, the determination of chlorophyll and chlorophyll fluorescence dynamics parameters, The drought resistance was comprehensively analyzed by subordinate function method.The results showed that Under water stress, the SPAD value, the maximum fluorescence (Fm), the potential photochemical efficiency (Fv/F0) and the maximum photochemical efficiency (Fv/Fm) of sugarcane were all decreased, and the initial fluorescence (F0) increased except YunRui15-58.It was suggested that water stress damage the light system II (PSII), which makes the potential photochemical efficiency (Fv/F0) and the maximum photochemical efficiency (Fv/Fm) reduced, and the photoelectron transfer was blocked;Grey correlation analysis showed that the correlation between 5 photosynthetic indexes and drought tolerance of sugarcane was not consistent under moderate and severe water stress.Under moderate water stress, the close correlation between the indexes and the drought tolerance of sugarcane was F0 > Fv/Fm > SPAD value > Fv/F0 > Fm, and the correlation coefficients were 0.8649, 0.6664, 0.6582, 0.6006 and 0.5952, respectively.Under severe water stress, the correlation degree was SPAD value > Fv/F0> Fm > Fv/Fm > F0, and the correlation coefficient were 0.9290, 0.8829, 0.8473, 0.7636, and 0.6326, respectively. The correlation analysis showed that the SPAD value was positively correlated with the potential photochemical efficiency (Fv/F0), negatively correlated with the other 3 indexes, and the initial fluorescence (F0 ) was positively correlated with the maximum fluorescence (Fm) and the maximum photochemical efficiency (Fv/Fm).The potential photochemical efficiency (Fv/F0) was negatively correlated with the maximum photochemical efficiency (Fv/Fm).The changes of chlorophyll fluorescence parameters and chlorophyll content of the 4 samples were different, and the adaptability of the whole treatment to water stress in the whole treatment was YunRui 15-80 > YunRui 15-84 > YunRui 15-85> YunRui 15-79.

References

[1]须晖, 高洁, 王蕊,等. 番茄幼苗叶绿素荧光参数对水分胁迫的响应[J]. 中国农学通报, 2011, 27(10):189-193.
[2] 李杨瑞. 现代甘蔗学[M]. 中国农业出版社, 2010.
[3] 苏坎坛, 杨焜正, 黄绍英,等. 土壤水分对甘蔗生理功能、产量和蔗糖份的影响[J]. 福建农业学报, 1988(1).
[4] 陈少裕. 水分胁迫对甘蔗生长发育及其产量和品质的影响综述[J]. 福建甘蔗, 1991(3):7-10.
[5] Jeyaramrajaa P R, Meenakshi S N, Kumar R S, et al. Water deficit induced oxidative damage in tea (Camellia sinensis) plants.[J]. Journal of Plant Physiology, 2005, 162(4):413-419.
[6] 莫伟平, 严婷婷, 崔春梅,等. 水分胁迫对巨峰葡萄叶片PSⅡ光化学效率及光能分配的影响[J]. 中国农业大学学报, 2015, 20(3):59-64.
[7] 陈歆, 刘贝贝, 韩丙军,等. 土壤水分胁迫对槟榔幼苗叶绿素荧光动力学参数的影响[J]. 热带作物学报, 2012, 33(2):230-237.
[8] 刘家勇, 陈学宽, 夏红明,等. 干旱胁迫下气温对甘蔗叶绿素荧光动力学参数的影响[J]. 西南农业学报, 2018(3).
[9] 白志英, 李存东, 赵金锋,等. 干旱胁迫对小麦代换系叶绿素荧光参数的影响及染色体效应初步分析[J]. 中国农业科学, 2011, 44(1):47-57.
[10] 陈建明, 俞晓平, 程家安. 叶绿素荧光动力学及其在植物抗逆生理研究中的应用[J]. 浙江农业学报, 2006, 18(1):51-55.
[11] 张永强, 李文杰. 干旱胁迫对冬小麦叶绿素荧光的影响[J]. 中国生态农业学报, 2002, 10(4):13-15.
[12]吴金芝, 王志敏, 李友军, 冯伟森, 田文忠, 高海涛. 不同冬小麦品种旗叶叶绿素荧光特性及其对干旱胁迫的响应[J]. 麦类作物学报, 2015, 35(5):699-706.
[13] 高丽丽, 李淦, 康正华,等. 脱叶剂对棉花叶片叶绿素荧光动力学参数的影响[J]. 棉花学报, 2016, 28(4):345-352.
[14] 李泽, 谭晓风, 卢锟,等. 根外追肥对油桐幼苗生长、光合作用及叶绿素荧光参数的影响[J]. 中南林业科技大学学报, 2016, 36(2):40-44.
[15] 王一, 张霞, 杨文钰,等. 不同生育时期遮阴对大豆叶片光合和叶绿素荧光特性的影响[J]. 中国农业科学, 2016, 49(11):2072-2081.
[16] 马瑞丽, 胥生荣, 陈垣,等. 干旱胁迫对黄芪叶片光合特性和叶绿素荧光参数的影响[J]. 中药材, 2018(3).
[17] 方志存, 刀静梅, 樊仙等. 甘蔗叶片SPAD值与含氮量及相关分析[J]. 福建农业学报, 2015(6):662-624.
[18] 张守仁. 叶绿素荧光动力学参数的意义及讨论[J]. 植物学报, 1999, 16(4):444-448.
[19] 唐启义.DPS数据处理系统[M].北京:科学出版社,2010:93-95.
[20] 刘志梅, 蒋文伟, 杨广远,等. 干旱胁迫对3种金银花叶绿素荧光参数的影响[J]. 浙江农林大学学报, 2012, 29(4):533-539.
[21] 韩苗苗, 张仁和, 朱永波,等. 不同玉米品种对干旱胁迫的响应[J]. 种子, 2008, 27(10):49-51.
[22] 许大全, 沈允钢. 光合作用的限制因素[M]. 北京：植物生理与分子生物学科学出版社,1997.
[23] 吴甘霖, 段仁燕, 王志高,等. 干旱和复水对草莓叶片叶绿素荧光特性的影响[J]. 生态学报, 2010, 30(14):3941-3946.
[24] 史胜青, 袁玉欣, 张金香,等. 不同水分胁迫方式对核桃苗叶绿素荧光动力学特性的影响[J]. 河北农业大学学报, 2003, 26(2):20-24.
[25] 宫丽丹, 魏丽萍, 倪书邦,等. 持续干旱对油棕幼苗叶绿素荧光动力学参数的影响[J]. 中国农学通报, 2016, 32(13):1-6.
[26] 付凤玲, 李晚忱, 潘光堂. 模糊隶属法对玉米苗期耐旱性的拟合分析[J]. 干旱地区农业研究, 2003, 21(1):83-85.
[27] 边芯, 董立华, 孙有芳,等. 云南割手密及其血缘 F1代材料抗旱相关性状的主成分分析[J]. 干旱地区农业研究, 2014, 32(3):56-61.
[28] 田春艳, 桃联安, 俞华先,等. 5种气候生态型割手密F1和F2杂种的耐旱性评价[J]. 中国农业科学, 2017, 50(22):4408-4421.
[29] 张云华, 王荣富, 阮龙. 水分胁迫对甘薯叶绿素荧光和光合特性的影响[J]. 中国农学通报, 2005, 21(8):208-210.
[30] 罗俊, 张木清, 吕建林,等. 水分胁迫对不同甘蔗品种叶绿素a荧光动力学的影响[J]. 福建农林大学学报(自然科学版), 2000, 29(1):18-22.
[31] 张国盛, 郝蕾, 闫子娟,等. 6种树种叶片叶绿素荧光动力学参数对土壤水分变化的响应[J]. 生态学杂志, 2017, 36(11):3079-3085.
[32] 王艺陶, 周宇飞, 李丰先,等. 干旱胁迫对高粱叶绿素荧光参数的影响[J]. 沈阳农业大学学报, 2013, 44(4):398-403.
[33] 胡学华, 蒲光兰, 肖千文,等. 水分胁迫下李树叶绿素荧光动力学特性研究[J]. 中国生态农业学报, 2007, 15(1):75-77.
[34] 张金政, 张起源, 孙国峰,等. 干旱胁迫及复水对玉簪生长和光合作用的影响[J]. 草业学报, 2014, 23(1):167-176.