Abstract: To study the association between late spring cold and stigma and pollen vigor of different wheat varieties in the Huang-huai winter wheat region, the leading wheat varieties were used as female parent and male parent in hybridization, sexual hybridization experiments were conducted between five winter wheat varieties after emasculation 1, 3, 5, 7 and 9 days, the hybrid seed rate of different combinations were studied after harvest, which represent stigma vitality and pollen activity of the wheat varieties. The results showed that when 5 wheat varieties were used as female parents, the order of average seed setting rate was‘Zhoumai 18’＞‘Bainong 207’＞‘Xinong 979’＞‘Liangxing 99’＞‘Yanshi 4110’, the average seed setting rate of‘Zhoumai 18’was 72.13%, and showed the highest stigma vitality; there was no significant difference between‘Bainong 207’and‘Zhoumai 18’, and the average seed setting rate of‘Zhoumai 18’was significantly higher than that of the other three varieties. When the 5 wheat varieties were used as male parents, the order of average seed setting rate was‘Bainong 207’＞‘Zhoumai 18’＞‘Yanshi 4110’＞ ‘Xinong 979’＞‘Liangxing 99’, the average seed setting rate of‘Bainong 207’was 72.00%, and showed the highest pollen activity, the difference between‘Bainong 207’and‘Zhoumai 18’was not significant, but the average seed setting rate of‘Bainong 207’was significantly higher than that of the other three varieties.