Effects of LED Spectrum Treatments at in vitro Culture Stage on the Growth and Minituber Production of Potato Plantlets After Transplanting in Greenhouse

doi:10.11924/j.issn.1000-6850.casb18110023

Abstract

Abstract:

The morphology of potato plantlets grown in vitro under different LED light sources varies obviously. To explore whether the variations will have lasting effects on potato plantlets’ growth after transplanting in greenhouse, a single-factor randomized block design test was conducted with 4-week-old potato plantlets grown in vitro under 100% red light (R), 100% blue light (B), 100% green light (G), 75% red light+ 25% blue light (RB), 45% red light+ 35% blue light+ 20% green light (RBG) and 100% white (CK) light spectrum being transplanted in greenhouse to observe their growth and tuberization performance. The results indicated that chlorophyll fluorescence Fv/Fm and qP, SPAD value and biomass accumulation of potato plantlets from B treatment were always higher than that of CK and other treatments; the biggest minituber dry weight and fresh weight and the proportion of effective minituber in B treatment were increased by 45.66%, 45.09% and 10.93%, respectively, compared with CK. R treatment increased the number of minituber per plantlet by 29.60% but decreased the proportion of effective minituber by 63.97%. The parameters including Fv/Fm, qP, qN, SPAD, biomass accumulation in different organs and minituber yield and yield composition of plantlets from combined spectrum RB and RBG showed no significant differences from that of CK. However, in terms of both growth and tuberization, plantlets from G treatment performed the worst after transplanting. Thus, the effects of monochromic red, blue and green LED spectrum on the growth and tuberization of potato plantlets grown in vitro continued after the plantlets’ transplanting in greenhouse, whereas the combined spectrum RB and RBG did not. Thus, in the cultivation of potato plantlets in vitro, monochromatic blue and red LED spectrum have potential application value as light sources to provide illumination and combined spectrum RB and RBG can be utilized as the alternatives of white light.

Key words: LED spectrum, tissue cultured potato plantlets, minituber production, potato plant growth

CLC Number:

S144.1

Chen Lili, Wang Haoying, Gong Xiaochen, Xue Xuzhang, Hu Yuegao. Effects of LED Spectrum Treatments at in vitro Culture Stage on the Growth and Minituber Production of Potato Plantlets After Transplanting in Greenhouse[J]. Chinese Agricultural Science Bulletin, 2020, 36(6): 11-18.

Figures/Tables 6

References 25

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日期	处理	植株鲜重	植株干重	叶片鲜重	叶片干重	茎秆鲜重	茎秆干重	地下鲜重	地下干重
10月15日	R	9.13±0.59b	0.96±0.01b	4.69±0.12b	0.52±0.01b	3.36±0.12b	0.31±0.01b	1.08±0.01ab	0.20±0.01ab
	B	16.3±0.98a	1.68±0.01a	7.87±0.14a	0.78±0.00a	6.35±0.23a	0.49±0.01a	2.16±0.28a	0.41±0.01a
	G	8.37±0.34b	0.72±0.01b	3.79±0.11b	0.34±0.01b	3.99±0.32b	0.25±0.01b	0.60±0.03b	0.12±0.01b
	CK	10.2±0.87b	0.96±0.01b	4.34±0.17b	0.39±0.01b	4.37±0.28b	0.29±0.01b	1.52±0.02ab	0.28±0.01ab
	RB	8.58±0.57b	1.02±0.01b	3.78±0.15b	0.34±0.01b	3.74±0.27b	0.25±0.01b	1.06±0.01ab	0.20±0.01ab
	RBG	11.0±0.87b	1.09±0.01b	4.84±0.10b	0.46±0.01b	4.47±0.19b	0.30±0.01b	1.74±0.02ab	0.32±0.01ab
10月30日	R	16.46±1.02bc	2.02±0.16cd	5.54±0.21ab	0.35±0.01ab	5.34±0.41ab	0.29±0.01ab	5.58±0.54cd	0.89±0.01cd
	B	27.32±2.04a	3.66±0.12a	7.08±0.58a	0.59±0.01a	5.86±0.34a	0.37±0.01a	14.39±1.12a	2.71±0.01a
	G	9.48±0.98c	1.02±0.02d	3.14±0.38b	0.22±0.01b	2.77±0.39c	0.15±0.01c	3.58±0.43d	0.58±0.01d
	CK	21.55±2.27ab	2.85±0.15ab	6.30±0.28a	0.45±0.01ab	4.23±0.58bc	0.26±0.01b	11.02±2.15bc	2.08±0.34ab
	RB	17.38±1.58b	2.20±0.11bc	5.98±0.83ab	0.39±0.01ab	3,76±0.78bc	0.22±0.01bc	8.23±0.89bc	1.49±0.01bc
	RBG	21.05±2.06ab	2.80±0.21ab	5.83±0.24ab	0.45±0.01ab	3.80±0.56bc	0.24±0.01bc	11.43±1.12bc	2.10±0.01ab
11月15日	R	17.14±1.20bc	2.06±0.02cd	2.57±0.38bc	0.22±0.01cd	1.90±0.24c	0.12±0.01c	6.67±1.22c	1.30±0.23cd
	B	30.63±3.29a	5.71±0.32a	6.64±0.49a	0.58±0.01a	4.81±0.57a	0.38±0.01a	19.38±1.54a	4.48±0.27a
	G	9.10±1.12c	1.21±0.01d	1.76±0.21c	0.14±0.01d	1.78±0.24c	0.12±0.01c	5.56±0.78c	0.96±0.01d
	CK	22.80±2.34b	4.07±0.21bc	3.77±0.24b	0.34±0.01b	2.85±0.26b	0.21±0.01b	14.17±2.23ab	3.53±0.89ab
	RB	17.57±1.27bc	3.95±0.01bc	3.75±0.49b	0.31±0.01bc	2.59±0.35bc	0.18±0.01bc	10.23±1.23bc	2.46±0.97c
	RBG	22.49±2.13bc	4.40±0.12b	4.46±0.57ab	0.41±0.01ab	2.13±0.25bc	0.15±0.01bc	13.01±1.24ab	3.27±0.45ab

日期	处理	植株鲜重	植株干重	叶片鲜重	叶片干重	茎秆鲜重	茎秆干重	地下鲜重	地下干重
10月15日	R	9.13±0.59b	0.96±0.01b	4.69±0.12b	0.52±0.01b	3.36±0.12b	0.31±0.01b	1.08±0.01ab	0.20±0.01ab
	B	16.3±0.98a	1.68±0.01a	7.87±0.14a	0.78±0.00a	6.35±0.23a	0.49±0.01a	2.16±0.28a	0.41±0.01a
	G	8.37±0.34b	0.72±0.01b	3.79±0.11b	0.34±0.01b	3.99±0.32b	0.25±0.01b	0.60±0.03b	0.12±0.01b
	CK	10.2±0.87b	0.96±0.01b	4.34±0.17b	0.39±0.01b	4.37±0.28b	0.29±0.01b	1.52±0.02ab	0.28±0.01ab
	RB	8.58±0.57b	1.02±0.01b	3.78±0.15b	0.34±0.01b	3.74±0.27b	0.25±0.01b	1.06±0.01ab	0.20±0.01ab
	RBG	11.0±0.87b	1.09±0.01b	4.84±0.10b	0.46±0.01b	4.47±0.19b	0.30±0.01b	1.74±0.02ab	0.32±0.01ab
10月30日	R	16.46±1.02bc	2.02±0.16cd	5.54±0.21ab	0.35±0.01ab	5.34±0.41ab	0.29±0.01ab	5.58±0.54cd	0.89±0.01cd
	B	27.32±2.04a	3.66±0.12a	7.08±0.58a	0.59±0.01a	5.86±0.34a	0.37±0.01a	14.39±1.12a	2.71±0.01a
	G	9.48±0.98c	1.02±0.02d	3.14±0.38b	0.22±0.01b	2.77±0.39c	0.15±0.01c	3.58±0.43d	0.58±0.01d
	CK	21.55±2.27ab	2.85±0.15ab	6.30±0.28a	0.45±0.01ab	4.23±0.58bc	0.26±0.01b	11.02±2.15bc	2.08±0.34ab
	RB	17.38±1.58b	2.20±0.11bc	5.98±0.83ab	0.39±0.01ab	3,76±0.78bc	0.22±0.01bc	8.23±0.89bc	1.49±0.01bc
	RBG	21.05±2.06ab	2.80±0.21ab	5.83±0.24ab	0.45±0.01ab	3.80±0.56bc	0.24±0.01bc	11.43±1.12bc	2.10±0.01ab
11月15日	R	17.14±1.20bc	2.06±0.02cd	2.57±0.38bc	0.22±0.01cd	1.90±0.24c	0.12±0.01c	6.67±1.22c	1.30±0.23cd
	B	30.63±3.29a	5.71±0.32a	6.64±0.49a	0.58±0.01a	4.81±0.57a	0.38±0.01a	19.38±1.54a	4.48±0.27a
	G	9.10±1.12c	1.21±0.01d	1.76±0.21c	0.14±0.01d	1.78±0.24c	0.12±0.01c	5.56±0.78c	0.96±0.01d
	CK	22.80±2.34b	4.07±0.21bc	3.77±0.24b	0.34±0.01b	2.85±0.26b	0.21±0.01b	14.17±2.23ab	3.53±0.89ab
	RB	17.57±1.27bc	3.95±0.01bc	3.75±0.49b	0.31±0.01bc	2.59±0.35bc	0.18±0.01bc	10.23±1.23bc	2.46±0.97c
	RBG	22.49±2.13bc	4.40±0.12b	4.46±0.57ab	0.41±0.01ab	2.13±0.25bc	0.15±0.01bc	13.01±1.24ab	3.27±0.45ab

处理	薯块分级占比/%		结薯数目/(粒/株)	最大薯鲜重/g	最大薯干重/g	单株薯鲜重/(g/株)	单株薯干重/(g/株)
处理	<20 mm	>20 mm	结薯数目/(粒/株)	最大薯鲜重/g	最大薯干重/g	单株薯鲜重/(g/株)	单株薯干重/(g/株)
R	79.08±2.23a	20.92±2.23c	3.42±0.67a	3.32±0.84c	0.96±0.02c	5.14±0.68c	1.98±0.25c
B	4.18±1.46d	95.82±1.46a	2.24±0.54b	9.17±1.23a	3.19±0.32a	13.94±1.01b	3.06±0.35b
G	82.12±4.89a	17.88±4.89d	2.03±0.71b	3.13±0.86c	0.82±0.25c	4.35±0.47d	1.02±0.07d
CK	15.11±3.47b	84.89±3.47b	2.50±0.59b	6.32±1.48b	2.19±0.93b	17.57±1.84ab	4.46±0.82a
RB	16.27±3.53b	83.73±3.53b	2.45±0.81b	5.36±1.19b	2.05±0.26b	16.81±2.17ab	3.87±0.74ab
RBG	12.79±4.09c	87.21±4.09b	2.33±0.92b	5.25±1.06b	2.08±0.52b	18.86±2.01a	4.98±0.85a

处理	薯块分级占比/%		结薯数目/(粒/株)	最大薯鲜重/g	最大薯干重/g	单株薯鲜重/(g/株)	单株薯干重/(g/株)
处理	<20 mm	>20 mm	结薯数目/(粒/株)	最大薯鲜重/g	最大薯干重/g	单株薯鲜重/(g/株)	单株薯干重/(g/株)
R	79.08±2.23a	20.92±2.23c	3.42±0.67a	3.32±0.84c	0.96±0.02c	5.14±0.68c	1.98±0.25c
B	4.18±1.46d	95.82±1.46a	2.24±0.54b	9.17±1.23a	3.19±0.32a	13.94±1.01b	3.06±0.35b
G	82.12±4.89a	17.88±4.89d	2.03±0.71b	3.13±0.86c	0.82±0.25c	4.35±0.47d	1.02±0.07d
CK	15.11±3.47b	84.89±3.47b	2.50±0.59b	6.32±1.48b	2.19±0.93b	17.57±1.84ab	4.46±0.82a
RB	16.27±3.53b	83.73±3.53b	2.45±0.81b	5.36±1.19b	2.05±0.26b	16.81±2.17ab	3.87±0.74ab
RBG	12.79±4.09c	87.21±4.09b	2.33±0.92b	5.25±1.06b	2.08±0.52b	18.86±2.01a	4.98±0.85a