Distribution Characteristics of Rhizosphere AM Fungi of Four Common Plants of Evergreen Broad-leaved Forests of Lushan Mountain in Summer

doi:10.11924/j.issn.1000-6850.casb2022-0020

Abstract

Abstract:

To explore the distribution characteristics of soil arbuscular mycorrhizal fungi (AM fungi), and promote the development and utilization of underground AM fungi resources, the distribution characteristics of rhizosphere AM fungal communities of four common plants (Symplocos stellaris, Loropetalum chinense, Camellia fraternal and Lindera glauca) of typical evergreen broad-leaved forests of Lushan Mountain in summer were investigated according to the method of spore morphology classification. The results showed that 44 AM fungal species (4 dominant species, 5 most common species, 12 common species, and 23 rare species) were isolated and identified. (1) There were significant differences in root colonization rate among different plants. The root colonization rate was positively correlated with the spore density and species richness of AM fungi in dominant and most common species, while negatively correlated with them in common and rare species, and the correlations were both not significant. (2) There was no significant difference in total spore density of AM fungi, while significant differences were found in species richness of AM fungi among different plants. In addition, there was no significant difference in the spore density of AM fungi of common species and the species richness of AM fungi of most common and rare species among different plants. (3) A negative correlation between spore density and species richness was found in AM fungi with different dominance. There was only a significantly negative correlation between the spore density of dominant species AM fungi and the spore density of common and rare species AM fungi. (4) Soil nutrients (total nitrogen, total phosphorus, total potassium, ammoniacal nitrogen, and nitrate nitrogen), pH and soil water contributed less to the distribution of AM fungi. However, the distribution of AM fungi was mainly affected by soil nitrogen. AM fungi of various dominant degrees were affected differently by soil nutrients, pH and water (rare species > common species > most common species > dominant species). The symbiosis pattern between common plants and AM fungi might be dominated by dominant and most common species, and supplemented by common and rare species of evergreen broad-leaved forests of Lushan Mountain in summer. According to the symbiosis pattern, AM fungi resources of plant rhizosphere were abundant and unevenly distributed; the degree of symbiosis between AM fungi and plants was determined by the different dominance of AM fungi; the interaction between AM fungi with different dominance was small; AM fungi were less affected by soil nutrients (total nitrogen, ammonia nitrogen, nitrate nitrogen, total phosphorus, and total potassium), pH and water, however, AM fungi of various dominant degrees were affected differently by soil nutrients, pH value and water.

Key words: Lushan Mountain, evergreen broad-leaved forest, AM fungi community, summer, common plants

WU Jiahai, ZOU Qin, DU Qu, ZHANG Tao, LI Jinmiao, XIAO Bin, LIU Wei. Distribution Characteristics of Rhizosphere AM Fungi of Four Common Plants of Evergreen Broad-leaved Forests of Lushan Mountain in Summer[J]. Chinese Agricultural Science Bulletin, 2022, 38(36): 48-55.

Figures/Tables 8

References 37

[1]	刘信中, 王琅. 江西庐山自然保护区生物多样性考察与研究[M]. 北京: 科学出版社, 2010:3-293.
[2]	万慧琳, 冯宗炜. 庐山常绿阔叶林物种组成及其演替趋势[J]. 生态学报, 2008, 28(3):1147-1157.
[3]	罗巧玉, 王晓娟, 李媛媛, 等. AM真菌在植物病虫害生物防治中的作用机制[J]. 生态学报, 2013(19):5997-6005.
[4]	王瑾, 毕银丽, 邓穆彪, 等. 丛枝菌根对采煤沉陷区紫穗槐生长及土壤改良的影响[J]. 科技导报, 2014, 32(11):26-32.
[5]	高萍, 李芳, 郭艳娥, 等. 丛枝菌根真菌和根瘤菌防控植物真菌病害的研究进展[J]. 草地学报, 2017, 25(2):236-242. doi: 10.11733/j.issn.1007-0435.2017.02.003
[6]	SMITH S E, READ D J. Mycorrhizal symbiosis(3nd edn)[M]. San Diego: Academic Cambridge, 2008.
[7]	LOVELOCK C E, MORTON A J B. Arbuscular mycorrhizal communities in tropical forests are affected by host tree species and environment[J]. Oecologia, 2003, 135(2):268-279. pmid: 12698349
[8]	GEHRING C A, CONNELL J H. Arbuscular mycorrhizal fungi in the tree seedlings of two Australian rain forests:occurrence,colonization, and relationships with plant performance[J]. Mycorrhiza, 2006, 16(2):89-98. doi: 10.1007/s00572-005-0018-5 URL
[9]	SHARMAH D, JHA D K. Diversity of arbuscular mycorrhizal fungi in disturbed and undisturbed forests of Karbi Anglong Hills of Assam,India[J]. Agricultural Research, 2014, 3(3):229-238. doi: 10.1007/s40003-014-0110-1 URL
[10]	石兆勇, 刘德鸿, 王发园, 等. 菌根类型对森林树木净初级生产力的影响[J]. 生态环境学报, 2012, 21(03):404-408.
[11]	刘永俊, 冯虎元. 丛枝菌根真菌系统分类及群落研究技术进展[J]. 应用生态学报, 2010, 21(6):1573-1580.
[12]	史立君, 于建新, 陈应龙, 等. 城市生态系统中丛枝菌根真菌侵染状况及群落特征[J]. 菌物学报, 2019, 38(11):2016-2019.
[13]	尚昆, 石磊, 李海波, 等. 梵净山不同海拔丛枝菌根真菌多样性[J]. 东北林业大学学报, 2020, 48(2):76-80.
[14]	AN Z Q, HENDRIX J W, HENSON D E H T. Evaluation of the ‘most probable number’ (MPN) and wet-sieving methods for determining soil-borne populations of endogonaceous mycorrhizal fungi[J]. Mycologia, 1990, 82:516-581.
[15]	吴佳海, 孔凡前, 刘苑秋, 等. 庐山常绿落叶阔叶混交林土壤AM真菌资源及其分布特征[J]. 南方农业学报, 2021, 52(8):2183-2192.
[16]	王幼珊, 刘润进. 球囊菌门丛枝菌根真菌最新分类系统菌种名录[J]. 菌物学报, 2017, 36(7):820-850.
[17]	钟凯, 袁玉清, 赵洪海, 等. 泰山丛枝菌根真菌群落结构特征[J]. 菌物学报, 2010, 29(1):44-50.
[18]	董怡然, 张秀英, 陈熙, 等. 福建省自然分布的野鸦椿根围土壤中丛枝菌根真菌多样性分析[J]. 植物资源与环境学报, 2018, 27(3):79-86.
[19]	春蕾, 峥嵘. 柄扁桃根围土AMF的季节变化及与土壤因子的关系[J]. 西北农林科技大学学报:自然科学版, 2017, 45(9):63-70.
[20]	鲍士旦. 土壤农化分析(第三版)[M]. 北京: 中国农业出版社, 2010.
[21]	MCGONIGLE T P, MILLER M H, EVANS D G, et al. A new method which gives an objective measure of colonization of roots by vesicular-arbuscular mycorrhizal fungi[J]. New phytologist, 1990, 115:492-501.
[22]	VIERHEILIG H, COUGHLAN A P, WYSS U, et al. Ink and vinegar,a simple staining technique for arbuscular mycorrhizal fungi[J]. Applied and environmental microbiology, 1998, 64(12):5004-5007. doi: 10.1128/AEM.64.12.5004-5007.1998 URL
[23]	胡从从, 成斌, 王坤, 等. 蒙古沙冬青及其伴生植物AM真菌物种多样性[J]. 生态学报, 2017, 37(23):7972-7982.
[24]	VAN DER HEIJDEN M G A, HORTON T R. Socialism in soil? The importance of mycorrhizal fungal networks for facilitation in natural ecosystems[J]. Journal of ecology, 2009, 97(6):1139-1150. doi: 10.1111/j.1365-2745.2009.01570.x URL
[25]	张中峰, 张金池, 黄玉清, 等. 接种菌根真菌对青冈栎幼苗耐旱性的影响[J]. 生态学报, 2016, 36(11):3402-3410.
[26]	姜攀, 王明元. 厦门市七种药用植物根围AM真菌的侵染率和多样性[J]. 生态学报, 2012, 32(13):4043-4051.
[27]	宰学明, 郝振萍, 张焕仕, 等. 傅家边丘陵山地滨梅根围AM真菌与土壤酶活性的关系[J]. 植物科学学报, 2013, 31(2):107-113.
[28]	贺学礼, 杨静, 赵丽莉. 荒漠沙柳根围AM真菌的空间分布[J]. 生态学报, 2011, 31(8):2159-2168.
[29]	向丹, 徐天乐, 李欢, 等. 丛枝菌根真菌的生态分布及其影响因子研究进展[J]. 生态学报, 2017, 37(11):3597-3606.
[30]	袁腾, 陶光耀, 江龙. 梵净山4种林型的土壤丛枝菌根真菌多样性[J]. 东北林业大学学报, 2018, 46(3):83-86.
[31]	BONFIM J A, VASCONCELLOS R L F, GUMIERE T, et al. Diversity of arbuscular mycorrhizal fungi in a Brazilian Atlantic Forest Toposequence[J]. Microbial ecology, 2016, 71:164-177. doi: 10.1007/s00248-015-0661-0 pmid: 26304552
[32]	DANIELL T J, HUSBAND R, FITTER A H, et al. Molecular diversity of arbuscular mycorrhizal fungi colonising arable crops[J]. Fems microbiology ecology, 2001(2-3):203-209. pmid: 11451525
[33]	陈志超, 石兆勇, 田长彦, 等. 古尔班通古特沙漠南缘短命植物根际AM真菌群落特征研究[J]. 菌物学报, 2008, 27(5):663-672.
[34]	何斐, 李冬花, 卜凡. 不同品种茶树根际AM真菌群落结构分析[J]. 茶叶科学, 2020, 40(3):319-327.
[35]	PORRAS-ALFARO A, HERRERA J, NATVIG D O, et al. Effect of long-term nitrogen fertilization on mycorrhizal fungi associated with a dominant grass in a semiarid grassland[J]. Plant soil, 2007, 296:65-75. doi: 10.1007/s11104-007-9290-9 URL
[36]	DIEPEN L T A V, LILLESKOV E A, PREGITZER K S. Simulated nitrogen deposition affects community structure of arbuscular mycorrhizal fungi in northern hardwood forests[J]. Molecular ecology, 2011, 20(4):799-811. doi: 10.1111/j.1365-294X.2010.04969.x pmid: 21210962
[37]	XU X H, CHEN C, ZHANG Z, et al. The influence of environmental factors on communities of arbuscular mycorrhizal fungi associated with Chenopodium ambrosioides revealed by MiSeq sequencing investigation[J]. Scientific reports, 2017, 7(1):45134. doi: 10.1038/srep45134 URL

菌种	相对多度	分离频率	优势度
无梗囊霉12	0.36	2.86	3.22
无梗囊霉13	0.54	4.29	4.83
无梗囊霉16	0.18	1.43	1.61
无梗囊霉17	0.18	1.43	1.61
无梗囊霉18	0.36	2.86	3.22
无梗囊霉19	0.18	1.43	1.61
无梗囊霉20	0.18	1.43	1.61
凹坑无梗囊霉	3.78	25.71	29.49
孔窝无梗囊霉	3.42	21.43	24.85
疏线无梗囊霉	0.36	2.86	3.22
双网无梗囊霉	0.18	1.43	1.61
细凹无梗囊霉	4.32	27.14	31.46
细齿无梗囊霉	5.04	28.57	33.61
细点无梗囊霉	5.40	37.14	42.54
疣状无梗囊霉	3.06	22.86	25.91
瑞氏无梗囊霉	0.36	2.86	3.22
球囊霉1	7.37	45.71	53.09
球囊霉2	6.65	42.86	49.51
球囊霉3	2.88	18.57	21.45
球囊霉4	1.44	11.43	12.87
球囊霉5	7.55	48.57	56.13
球囊霉6	9.17	50.00	59.17
球囊霉7	6.29	35.71	42.01
球囊霉8	2.34	17.14	19.48
球囊霉9	0.36	2.86	3.22
球囊霉10	1.44	10.00	11.44
球囊霉14	2.52	12.86	15.38
球囊霉18	0.18	1.43	1.61
布氏球囊霉	9.71	50.00	59.71
大果球囊霉	1.08	7.14	8.22
富氏球囊霉	0.90	5.71	6.61
小果球囊霉	1.26	8.57	9.83
荫性球囊霉	2.34	15.71	18.05
地表球囊霉	0.18	1.43	1.61
弯丝球囊霉	0.72	5.71	6.43
幼套近明球囊霉	0.36	2.86	3.22
根孢囊霉1	0.18	1.43	1.61
悬钩子硬囊霉	0.18	1.43	1.61
象牙白多样孢囊霉	0.90	7.14	8.04
巴西双型囊霉	0.72	5.71	6.43
薄壁双型囊霉	0.18	1.43	1.61
附柄双型囊霉	1.44	10.00	11.44
格拉纳达双型囊霉	2.16	14.29	16.44
詹氏双型囊霉	1.62	12.86	14.48

菌种	相对多度	分离频率	优势度
无梗囊霉12	0.36	2.86	3.22
无梗囊霉13	0.54	4.29	4.83
无梗囊霉16	0.18	1.43	1.61
无梗囊霉17	0.18	1.43	1.61
无梗囊霉18	0.36	2.86	3.22
无梗囊霉19	0.18	1.43	1.61
无梗囊霉20	0.18	1.43	1.61
凹坑无梗囊霉	3.78	25.71	29.49
孔窝无梗囊霉	3.42	21.43	24.85
疏线无梗囊霉	0.36	2.86	3.22
双网无梗囊霉	0.18	1.43	1.61
细凹无梗囊霉	4.32	27.14	31.46
细齿无梗囊霉	5.04	28.57	33.61
细点无梗囊霉	5.40	37.14	42.54
疣状无梗囊霉	3.06	22.86	25.91
瑞氏无梗囊霉	0.36	2.86	3.22
球囊霉1	7.37	45.71	53.09
球囊霉2	6.65	42.86	49.51
球囊霉3	2.88	18.57	21.45
球囊霉4	1.44	11.43	12.87
球囊霉5	7.55	48.57	56.13
球囊霉6	9.17	50.00	59.17
球囊霉7	6.29	35.71	42.01
球囊霉8	2.34	17.14	19.48
球囊霉9	0.36	2.86	3.22
球囊霉10	1.44	10.00	11.44
球囊霉14	2.52	12.86	15.38
球囊霉18	0.18	1.43	1.61
布氏球囊霉	9.71	50.00	59.71
大果球囊霉	1.08	7.14	8.22
富氏球囊霉	0.90	5.71	6.61
小果球囊霉	1.26	8.57	9.83
荫性球囊霉	2.34	15.71	18.05
地表球囊霉	0.18	1.43	1.61
弯丝球囊霉	0.72	5.71	6.43
幼套近明球囊霉	0.36	2.86	3.22
根孢囊霉1	0.18	1.43	1.61
悬钩子硬囊霉	0.18	1.43	1.61
象牙白多样孢囊霉	0.90	7.14	8.04
巴西双型囊霉	0.72	5.71	6.43
薄壁双型囊霉	0.18	1.43	1.61
附柄双型囊霉	1.44	10.00	11.44
格拉纳达双型囊霉	2.16	14.29	16.44
詹氏双型囊霉	1.62	12.86	14.48

AM真菌分布特征	老鼠矢	檵木	连蕊茶	山胡椒
孢子密度（个/10 g干土）	117.40±7.71a	113.30±4.20a	115.15±6.09a	118.15±7.12a
物种丰富度	6.90±0.41a	6.85±0.44a	6.55±0.29ab	5.44±0.73b
Shannon-Wiener指数	3.26
均匀度指数	0.53

AM真菌分布特征	老鼠矢	檵木	连蕊茶	山胡椒
孢子密度（个/10 g干土）	117.40±7.71a	113.30±4.20a	115.15±6.09a	118.15±7.12a
物种丰富度	6.90±0.41a	6.85±0.44a	6.55±0.29ab	5.44±0.73b
Shannon-Wiener指数	3.26
均匀度指数	0.53

土壤养分特征	老鼠矢	檵木	连蕊茶	山胡椒
pH	4.49±0.05a	4.53±0.06a	4.42±0.05a	4.51±0.05a
含水率/%	21.68±0.83b	23.65±1.00ab	21.53±0.45b	24.14±0.87a
全磷/(g/kg)	0.49±0.01a	0.49±0.02a	0.53±0.02a	0.52±0.01a
全钾/(g/kg)	11.94±0.19a	11.93±0.24a	12.34±0.23a	12.05±0.11a
氨态氮/(mg/kg)	13.00±1.57b	18.98±1.39ab	19.05±2.60a	17.99±1.09ab
硝态氮/(mg/kg)	2.68±0.64a	4.73±0.76a	4.28±0.89a	4.18±0.44a
全氮/(g/kg)	2.68±0.33ab	1.93±0.22b	3.13±0.36a	2.99±0.32a