Comparison of Combined Transformation Ability of Pleurotus pulmonarius and Protaetia brevitarsis Larvae to 11 Kinds of Forest Fruit Branch Waste

doi:10.11924/j.issn.1000-6850.casb2023-0412

Abstract

Abstract:

In order to explore the combined transformation ability of saprophytic edible fungus-Pleurotus pulmonarius and saprophytic insect- Protaetia brevitarsis larvae (PBL) to 11 kinds of forest and fruit tree branch waste, P. pulmonarius PL2 strain was used to biotransform different forest and fruit tree branches, and the mycelial growth rate, fruiting body yield, fruiting body polysaccharide content and antioxidant activity of fruiting body extract were determined. After that, the spent substrate of P. pulmonarius based on 11 kinds of wood branches was biotransformed by PBL, and the Hassan method was used to comprehensively evaluate the combined transformation effect of P. pulmonarius and PBL on 11 kinds of wood branches. The results showed that there were significant differences in the mycelial growth rate, fruiting body yield, fruiting body polysaccharide content and fruiting body antioxidant activity of P. pulmonarius strains among different types of branches. The mycelial growth rate was the fastest in beech wood (10.05 mm/d), the biological efficiency was the highest in honeysuckle branches (68.95%), the polysaccharide content was the highest in peach wood (9.52%), and the comprehensive antioxidant activity was the strongest in poplar wood (OD 0.6409). Further studies showed that there were significant differences in the combined transformation effect of P. pulmonarius and PBL on 11 kinds of branches. Among them, the combined transformation effect on honeysuckle branches was the best. 1 kg honeysuckle branches could produce 846.19 g of P. pulmonarius fruiting bodies. The weight gain of PBL was 45.97 g, and the amount of larvae frass production was 499.54 g. The results of this study showed that the combined conversion effect of P. pulmonarius and PBL on forest fruit tree branch waste was significant and had certain application value, which could provide theoretical basis for the ecological high-value utilization of forest fruit tree branch waste resources.

Key words: forest and fruit trees sawdust, Pleurotus pulmonarius, Protaetia brevitarsis larvae (PBL), biotransformation

ZHAO Wei, ZHAO Chuanyue, KONG Hao, XIE Xingtong, WANG Li. Comparison of Combined Transformation Ability of Pleurotus pulmonarius and Protaetia brevitarsis Larvae to 11 Kinds of Forest Fruit Branch Waste[J]. Chinese Agricultural Science Bulletin, 2024, 40(11): 134-141.

Figures/Tables 6

References 29

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栽培基质	菌丝生长速度/(mm/d)	生物学效率/%
桦木	9.15±0.44b	30.55±1.78cd
苹果木	8.00±0.16c	31.70±2.69cd
柞木	7.30±0.57d	31.40±1.48cd
榉木	10.05±0.72a	31.19±1.20cd
杨木	9.45±0.41ab	38.56±1.73cd
核桃木	9.95±0.34a	44.83±2.68bc
松木	6.25±0.50f	29.31±1.43d
桃木	7.00±0.43de	25.10±3.30d
板栗木	6.50±0.11ef	30.07±2.96d
金银花枝条	7.90±0.26c	68.95±4.99a
柠条	7.15±0.19d	55.17±6.98b

栽培基质	菌丝生长速度/(mm/d)	生物学效率/%
桦木	9.15±0.44b	30.55±1.78cd
苹果木	8.00±0.16c	31.70±2.69cd
柞木	7.30±0.57d	31.40±1.48cd
榉木	10.05±0.72a	31.19±1.20cd
杨木	9.45±0.41ab	38.56±1.73cd
核桃木	9.95±0.34a	44.83±2.68bc
松木	6.25±0.50f	29.31±1.43d
桃木	7.00±0.43de	25.10±3.30d
板栗木	6.50±0.11ef	30.07±2.96d
金银花枝条	7.90±0.26c	68.95±4.99a
柠条	7.15±0.19d	55.17±6.98b

栽培基质	还原力	DPPH清除能力/%	羟基清除能力/%
核桃木	1.21±0.00e	80.92±0.54ef	90.38±0.49ab
柠条	1.26±0.00cd	92.69±0.20a	72.71±9.31f
板栗木	1.40±0.00a	86.66±0.18c	78.39±0.37d
杨木	1.26±0.02cd	84.67±0.10d	91.17±0.13ab
柞木	1.27±0.00c	82.33±0.73ef	93.71±0.38a
金银花枝条	1.28±0.02c	79.05±1.62g	81.56±1.20c
桦木	1.14±0.00g	85.31±0.10cd	87.83±0.59b
苹果木	1.02±0.00h	82.39±0.37ef	92.92±0.08ab
榉木	1.29±0.00bc	83.09±0.27e	91.90±0.40ab
松木	1.16±0.00f	81.57±0.61ef	90.98±0.82ab
桃木	1.24±0.00e	89.82±0.35b	75.47±1.18e

栽培基质	还原力	DPPH清除能力/%	羟基清除能力/%
核桃木	1.21±0.00e	80.92±0.54ef	90.38±0.49ab
柠条	1.26±0.00cd	92.69±0.20a	72.71±9.31f
板栗木	1.40±0.00a	86.66±0.18c	78.39±0.37d
杨木	1.26±0.02cd	84.67±0.10d	91.17±0.13ab
柞木	1.27±0.00c	82.33±0.73ef	93.71±0.38a
金银花枝条	1.28±0.02c	79.05±1.62g	81.56±1.20c
桦木	1.14±0.00g	85.31±0.10cd	87.83±0.59b
苹果木	1.02±0.00h	82.39±0.37ef	92.92±0.08ab
榉木	1.29±0.00bc	83.09±0.27e	91.90±0.40ab
松木	1.16±0.00f	81.57±0.61ef	90.98±0.82ab
桃木	1.24±0.00e	89.82±0.35b	75.47±1.18e

栽培基质	归一化
栽培基质	还原力	DPPH清除率	羟基清除率	OD值
杨木	0.6316	0.4120	0.8790	0.6409
桦木	0.7105	0.2962	0.9138	0.6402
柞木	0.6579	0.2405	1.0000	0.6328
板栗木	1.0000	0.5579	0.2705	0.6095
柠条	0.6316	1.0000	0.0000	0.5439
桃木	0.5789	0.7896	0.1314	0.5000
桦木	0.3158	0.4589	0.7200	0.4982
核桃木	0.5000	0.1371	0.8414	0.4928
松木	0.3684	0.1848	0.8700	0.4744
苹果木	0.0000	0.2449	0.9624	0.4024
金银花枝条	0.6842	0.0000	0.4214	0.3685