Abstract:

In order to screen out the best ratio medium for in vitro rapid propagation of Solanum habrochaites ‘LA1777’ leaves, the method of in vitro culture was used. In this study, the leaves of S. habrochaites ‘LA1777’ were used as materials, MS medium was used as the basic medium, and different hormone combinations were set up, the induction rate and growth status of callus, adventitious buds and adventitious roots were observed and counted separately. The results showed that the best medium formula for promoting leaf callus formation was MS+ 3 mg/L 6-BA+ 0.3 mg/L IAA, and the callus rate was 95.83%. The best medium for promoting adventitious bud differentiation was MS+ 3 mg/L 6-BA+ 0.3 mg/L IAA+ 3 mg/L AgNO₃, and the induction rate of adventitious buds was 50.00%. The best medium for rooting culture was MS+ 0.1 mg/L NAA, and the adventitious root induction rate was 94.44%. This study provides reference for germplasm conservation, rapid propagation and genetic transformation of S. habrochaites.

Key words: Solanum habrochaites, leaves, in vitro culture, callus differentiation, adventitious bud induction, rooting culture, plant regeneration