Cold Plasma Technology in Seed Treatment and Plant Growth: A Review

doi:10.11924/j.issn.1000-6850.casb2024-0419

Abstract

Abstract:

Cold plasma technology has gained significant traction in agriculture due to its advantages, including low operational temperature, green, residue-free, high efficiency, and remarkable effectiveness in seed treatment, as well as in activating the internal physiological and biochemical processes of plants to improve resistance and yield. This paper reviews the progress of cold plasma technology in seed disinfection, breaking seed dormancy to promote germination, enhancing plant resistance, and increasing yield, etc. Furthermore, this paper provides a comprehensive analysis of the primary active ingredients of this technology and elucidates their mechanisms of action at the physicochemical, physiological and biochemical levels. In view of the differences in physical, chemical and physiological effects of different treatment objects after discharge by different types of cold plasma equipment, the future research direction is proposed: optimizing and establishing cold plasma treatment system, and clarifying the long-term effect of cold plasma treatment on the phenotypic genetic changes of plant growth cycle.

Key words: cold plasma, seed treatment, stress resistance, biological effect, plasma-activated water, reactive oxygen species, reactive nitrogen species, dielectric barrier discharge

ZHAO Xinru, XING Congying, YE Zifan, LIU Zhenzhong, LIANG Ke. Cold Plasma Technology in Seed Treatment and Plant Growth: A Review[J]. Chinese Agricultural Science Bulletin, 2025, 41(10): 45-51.

Figures/Tables 1

References 63

[1]	COLE M B, AUGUSTIN M A, ROBERTSON M J, et al. The science of food security[J]. NPJ science of food, 2018, 2(1):14.
[2]	ARORA N K. Impact of climate change on agriculture production and its sustainable solutions[J]. Environmental sustainability, 2019, 2(2):95-96.
[3]	JANNI M, MAESTRI E, GULLì M, et al. Plant responses to climate change, how global warming may impact on food security: A critical review[J]. Frontiers in plant science, 2024,14:1297569.
[4]	HOU D. Sustainable soil management for food security[J]. Soil use and management, 2023, 39(1):1-7.
[5]	SU F, LIU Y, CHEN L, et al. Impact of climate change on food security in the central Asian countries[J]. Science China: earth sciences, 2024, 67(1):268-280.
[6]	SONG J S, KIM S B, RYU S, et al. Emerging plasma technology that alleviates crop stress during the early growth stages of plants: A review[J]. Frontiers in plant science, 2020,11:988.
[7]	DOMONKOS M, TICHÁ P, TREJBAL J, et al. Applications of cold atmospheric pressure plasma technology in medicine, agriculture and food industry[J]. Applied sciences-basel, 2021, 11(11):4809.
[8]	STOLERU V, BURLICA R, MIHALACHE G, et al. Plant growth promotion effect of plasma activated water on Lactuca sativa L. cultivated in two different volumes of substrate[J]. Scientific reports, 2020, 10(1):20920.
[9]	李德全, 陈昭斌. 等离子体技术在消毒中的应用进展[J]. 中国消毒学杂志, 2023, 40(4):295-299. doi: 10.11726/j.issn.1001-7658.2023.04.016
[10]	YAN D, SHERMAN J H, KEIDAR M. Cold atmospheric plasma, a novel promising anti-cancer treatment modality[J]. Oncotarget, 2017, 8(9):15977-15995. doi: 10.18632/oncotarget.13304 pmid: 27845910
[11]	李和平, 于达仁, 孙文廷, 等. 大气压放电等离子体研究进展综述[J]. 高电压技术, 2016, 42(12):3697-3727.
[12]	FRIDMAN G, FRIEDMAN G, GUTSOL A, et al. Applied plasma medicine[J]. Plasma processes and polymers, 2008, 5(6):503-533.
[13]	STAEHLKE S, OSTER P, SEEMANN S, et al. Laser structured dental zirconium for soft tissue cell occupation-importance of wettability modulation[J]. Materials, 2022, 15(3):732.
[14]	BRADFORD K J, DAHAL P, ASBROUCK J V, et al. The dry chain: Reducing postharvest losses and improving food safety in humid climates[J]. Trends in food science & technology, 2018,71:84-93.
[15]	ADHIKARI B, ADHIKARI M, GHIMIRE B, et al. Cold plasma seed priming modulates growth, redox homeostasis and stress response by inducing reactive species in tomato (Solanum lycopersicum)[J]. Free radical biology and medicine, 2020,156:57-69.
[16]	齐先科, 李淼, 李彩虹, 等. 介质阻挡放电低温等离子体处理对小麦种子活力及幼苗生理的影响[J]. 农业工程学报, 2024, 40(1):301-309.
[17]	BIAN J Y, GUO X Y, LEE D H, et al. Non-thermal plasma enhances rice seed germination, seedling development, and root growth under low-temperature stress[J]. Applied biological chemistry, 2024, 67(1):2.
[18]	XU F, CHEN H, CHEN C, et al. The mutagenic effect of cold plasma on Medicago sativa L.[J]. Free radical biology and medicine, 2024,223:18-29.
[19]	LI H P I, LI J, ZHAO X T, et al. Applications of cold atmospheric plasmas (CAPs) in agriculture: A brief review and the novel development of a radio-frequency CAP jet generator for plant mutation[J]. Plasma science and technology, 2022, 24(9):093001.
[20]	穆艳, 赵国庆, 赵巧巧, 等. 活化水灌溉在农业生产中的应用研究进展[J]. 农业资源与环境学报, 2019, 36(4):403-411.
[21]	XIANG Q, FAN L, LI Y, et al. A review on recent advances in plasma-activated water for food safety: Current applications and future trends[J]. Critical reviews in food science and nutrition, 2022, 62(8):2250-2268.
[22]	GUO Q, MENG Y R, QU G Z, et al. Improvement of wheat seed vitality by dielectric barrier discharge plasma treatment[J]. Bioelectromagnetics, 2018, 39(2):120-131. doi: 10.1002/bem.22088 pmid: 29027733
[23]	SAYAHI K, SARI A H, HAMIDI A, et al. Application of cold argon plasma on germination, root length, and decontamination of soybean cultivars[J]. BMC plant biology, 2024, 24(1):59. doi: 10.1186/s12870-024-04730-4 pmid: 38247007
[24]	LIU C C, CUI J F, ZHANG D, et al. Decontamination of infected plant seeds utilizing atmospheric gliding arc discharge plasma treatment[J]. Plasma science & technology, 2021, 23(10):105501.
[25]	AHMAD A, SRIPONG K, UTHAIRATANAKIJ A, et al. Decontamination of seed borne disease in pepper (Capsicum annuum L.) seed and the enhancement of seed quality by the emulated plasma technology[J]. Scientia horticulturae, 2022,291:110568.
[26]	PANNGOM K, LEE S H, PARK D H, et al. Non-thermal plasma treatment diminishes fungal viability and up-regulates resistance genes in a plant host[J]. Plos one, 2014, 9(6):e99300.
[27]	KANG M H, PENGKIT A, CHOI K, et al. Differential inactivation of fungal spores in water and on seeds by ozone and arc discharge plasma[J]. Plos one, 2015, 10(9):e0139263.
[28]	万强贵. 辉光放电等离子体对茄病镰刀菌的杀菌作用及机理研究[D]. 兰州: 甘肃农业大学, 2023.
[29]	王博华, 薛东, 董闪闪, 等. 介质阻挡放电等离子体对单增李斯特菌的杀灭效果及作用机制研究[J]. 轻工学报, 2023, 38(3):17-24.
[30]	MOTYKA-POMAGRUK A, DZIMITROWICZ A, ORLOWSKI J, et al. Implementation of a non-thermal atmospheric pressure plasma for eradication of plant pathogens from a surface of economically important seeds[J]. International journal of molecular sciences, 2021, 22(17):9256.
[31]	PULIGUNDLA P, KIM J W, MOK C. Effects of nonthermal plasma treatment on decontamination and sprouting of radish (Raphanus sativus L.) seeds[J]. Food and bioprocess technology, 2017,10:1093-1102.
[32]	崔鲁彬, 孙运金, 王雪莹, 等. 滑动弧放电等离子体对枯草芽孢杆菌的灭菌机理[J]. 农业工程, 2021, 11(1):62-68.
[33]	JOSHI S G, COOPER M, YOST A, et al. Nonthermal dielectric-barrier discharge plasma-induced inactivation involves oxidative DNA damage and membrane lipid peroxidation in Escherichia coli[J]. Antimicrobial agents and chemotherapy, 2011, 55(3):1053-1062. doi: 10.1128/AAC.01002-10 pmid: 21199923
[34]	HU Y, ZHAO T, ZOU L, et al. Molecular dynamics simulations of membrane properties affected by plasma ROS based on the GROMOS force field[J]. Biophysical chemistry, 2019,253:106214.
[35]	DEZEST M, BULTEAU A L, QUINTON D, et al. Oxidative modification and electrochemical inactivation of Escherichia coli upon cold atmospheric pressure plasma exposure[J]. Plos one, 2017, 12(3):e0173618.
[36]	SHEN J, CHENG C, FANG S, et al. Sterilization of Bacillus subtilis spores using an atmospheric plasma jet with argon and oxygen mixture gas[J]. Applied physics express, 2012, 5(3):36201.
[37]	BOURKE P, ZIUZINA D, HAN L, et al. Microbiological interactions with cold plasma[J]. Journal of applied microbiology, 2017, 123(2):308-324. doi: 10.1111/jam.13429 pmid: 28245092
[38]	唐思敏, 童晓红, 应杰政, 等. 近年我国水稻种子休眠与萌发调控机制研究进展[J]. 中国稻米, 2024, 30(4):1-6. doi: 10.3969/j.issn.1006-8082.2024.04.001
[39]	KHAMSEN N, ONWIMOL D, TEERAKAWANICH N, et al. Rice (Oryza sativa L.) seed sterilization and germination enhancement via atmospheric hybrid nonthermal discharge plasma[J]. ACS applied materials & interfaces, 2016, 8(30):19268-19275.
[40]	GURAGAIN R P, BANIYA H B, GURAGAIN D P, et al. Exploring the effects of non-thermal plasma pre-treatment on coriander (Coriander sativum L.) seed germination efficiency[J]. Heliyon, 2024, 10(7):e28763.
[41]	王敏, 杨思泽, 陈青云, 等. 大气压等离子体处理对黄瓜种子萌发及幼苗生长的影响[J]. 农业工程学报, 2007, 23(2):195-200.
[42]	FATELNIG L M M, CHANYALEW S, TADESSE M, et al. Seed priming with gas plasma-activated water in Ethiopia’s “orphan” crop tef (Eragrostis tef)[J]. Planta, 2024, 259(4):75.
[43]	ADHIKARI B, ADHIKARI M, PARK G. The effects of plasma on plant growth, development, and sustainability[J]. Applied sciences, 2020, 10(17):6045.
[44]	WASKOW A, GUIHUR A, HOWLING A, et al. RNA sequencing of Arabidopsis thaliana seedlings after non-thermal plasma-seed treatment reveals upregulation in plant stress and defense pathways[J]. International journal of molecular sciences, 2022, 23(6):3070.
[45]	SADHU S, THIRUMDAS R, DESHMUKH R R, et al. Influence of cold plasma on the enzymatic activity in germinating mung beans (Vigna radiate)[J]. LWT-food science and technology, 2017,78:97-104.
[46]	DEGUTYTĖ-FOMINS L, PAUŽAITĖ G, ŽŪKIENĖ R, et al. Relationship between cold plasma treatment-induced changes in radish seed germination and phytohormone balance[J]. Japanese journal of applied physics, 2020,59:SH1001.
[47]	ATTRI P, ISHIKAWA K, OKUMURA T, et al. Impact of seed color and storage time on the radish seed germination and sprout growth in plasma agriculture[J]. Scientific reports, 2021, 11(1):2539. doi: 10.1038/s41598-021-81175-x pmid: 33510231
[48]	TALBI S, ROMERO-PUERTAS M C, HERNÁNDEZ A, et al. Drought tolerance in a Saharian plant Oudneya africana: Role of antioxidant defences[J]. Environmental and experimental botany, 2015,111:114-126.
[49]	赵东晓, 杜建勋, 董亚茹, 等. 常压室温等离子体对NaCl胁迫下胡麻种子萌发及幼苗生理特性的影响[J]. 核农学报, 2018, 32(8):1466-1476. doi: 10.11869/j.issn.100-8551.2018.08.1466
[50]	李想, 张梦, 刘春增, 等. 等离子体处理对紫云英种子萌发和生理特性的影响[J]. 草业学报, 2023, 32(10):129-140. doi: 10.11686/cyxb2022437
[51]	GIERCZIK K, VUKUŠIĆ T, KOVÁCS L, et al. Plasma-activated water to improve the stress tolerance of barley[J]. Plasma processes and polymers, 2020, 17(3):1900123.
[52]	GAO X T, ZHANG A, HÉROUX P, et al. Effect of dielectric barrier discharge cold plasma on pea seed growth[J]. Journal of agricultural and food chemistry, 2019, 67(39):10813-10822. doi: 10.1021/acs.jafc.9b03099 pmid: 31490069
[53]	王虹, 阎君, 赵志勇, 等. 低温等离子体活化水对菠菜种子萌发及幼苗生长的影响[J]. 上海蔬菜, 2023(3):93-96.
[54]	CHALISE R, BHANDARI P, SHARMA S, et al. Enhancement of wheat yield by atmospheric pressure plasma treatment[J]. AIP advances, 2023, 13(6):65104.
[55]	RENÁTA Š, NICOLETTE V, MONIKA B, et al. Enhanced in situ activity of peroxidases and lignification of root tissues after exposure to non-thermal plasma increases the resistance of pea seedlings[J]. Plasma chemistry and plasma processing, 2021, 41(3):903-922.
[56]	KARMAKAR S, BILLAH M, HASAN M, et al. Impact of LFGD (Ar & Oxygen) plasma on seed surface, germination, plant growth, productivity and nutritional composition of maize (Zea mays L.)[J]. Heliyon, 2021, 7(3):e06458.
[57]	ATTRI P, KOGA K, OKUMURA T, et al. Green route for ammonium nitrate synthesis: Fertilizer for plant growth enhancement[J]. RSC advances, 2021, 11(46):28521-28529. doi: 10.1039/d1ra04441a pmid: 35478561
[58]	BAFOIL M, JEMMAT A, MARTINEZ Y, et al. Effects of low temperature plasmas and plasma activated waters on Arabidopsis thaliana germination and growth[J]. Plos one, 2018, 13(4):e0195512.
[59]	李平, 陶华杨, 彭超, 等. DBD等离子体与活化水对马铃薯生长特性及产量的影响[J]. 高电压技术, 2024, 50(4):1742-1751.
[60]	THAN H A Q, PHAM T H, NGUYEN D K V, et al. Non-thermal plasma activated water for increasing germination and plant growth of Lactuca sativa L.[J]. Plasma chemistry and plasma processing, 2022, 42(1):73-89.
[61]	PAŃKA D, JESKE M, ŁUKANOWSKI A, et al. Can cold plasma be used for boosting plant growth and plant protection in sustainable plant production?[J]. Agronomy-basel, 2022, 12(4):841.
[62]	NOCTOR G, REICHHELD J P, FOYER C H. ROS-related redox regulation and signaling in plants[J] Seminars in cell and developmental biology, 2018, 80(S1):3-12.
[63]	DING W, LIN L, ZHANG B, et al. OsKASI, a β-ketoacyl-[acyl carrier protein] synthase I, is involved in root development in rice (Oryza sativa L.)[J]. Planta, 2015, 242(1):203-213.