The Enhancement of Stress Resistance of Tea Plants by Herb Extract and Plant Essential Oil

doi:10.11924/j.issn.1000-6850.casb2021-0898

Abstract

Abstract:

To understand the influence of herb extract and plant essential oil on the stress resistance of tea plant and to develop environment-friendly technology to improve tea plant resistance to various stresses, in this study, herb ethanol extracts (from the fresh shoots of rosemary, perilla and mint ) and biotic plant essential oil (methyl salicylate oil and methyl dihydrojasmonate) were made into 0.1% water solution which was used as external treatment agent to be sprayed on tea plants (7500 L/hm² for each treatment, twice continuously) in early December. The results showed that after treatment, the content of amino acids in tea leaves was increased by 27.8%-55.6% and the content of polyphenols was increased by 3.93%-24.2%. The relative content of dialdehyde in tea leaves, except those treated by mint extract, was decreased by 9.2%-32.8%. The enzyme activity analysis showed that the polyphenol oxidase activity of tea leaves was increased by 1.89-4.35 times, the lipoxygenase activity was increased by 1.41-2.58 times, and the superoxide dismutase activity was increased by 1.98-15.7 times, respectively. The phenylalanine ammonia lyase (PAL) activity was decreased except the treatment by rosemary and perilla extract. The expression of CsOPB3 in tea leaves, a key gene of jasmonic acid signal pathway, was significantly up-regulated after treatment with dihydrojasmonate, rosemary and perilla extract, but down-regulated after treatment with peppermint extract and holly oil. The scavenging ability of water extract of fresh tea leaves treated with herb extract or plant essential oil to superoxide free radical was increased by 1.3-2.9 times. The study shows that spraying herb extract and plant essential oil in early winter tea garden can improve the adaptability of tea plants to low temperature. According to the common indicating role of physiological indexes measured in this study to evaluate tea plants against other abiotic stresses or plant diseases and insect pests, the results further reveal that different herb extracts and plant essential oils used in the study could have a certain application value for tea plants against more abiotic and biotic stresses.

Key words: plant essential oil, herb extract, tea plant, stress resistance

CLC Number:

S571.1

LI Ronglin, TANG Jun, AI Zeyi, MU Bing, YANG Yiyang, CHEN Zhengtao, SHI Haihua. The Enhancement of Stress Resistance of Tea Plants by Herb Extract and Plant Essential Oil[J]. Chinese Agricultural Science Bulletin, 2022, 38(26): 111-117.

Figures/Tables 4

References 40

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试验处理	多酚/% (冻干样）	黄酮总量/% （冻干样）	木质素/% （冻干样）	氨基酸/% （冻干样）	丙二醛/mg/kg （鲜叶）
对照	19.55±0.36a	3.30±0.01b	2.12±0.12a	1.67±0.35a	9.15±0.00a
冬青油	20.32±0.09a	2.63±0.78a	1.76±0.09b	2.34±0.36c	8.43±0.08a
二氢茉莉酸酯	24.28±0.37c	3.62±0.04b	1.96±0.10c	2.26±0.44bc	7.60±0.72b
迷迭香提取物	21.76±0.10b	2.73±0.33a	2.54±0.12d	2.60±0.19d	6.15±0.30c
紫苏提取物	23.19±0.39c	2.63±0.78a	2.32±0.10e	2.13±0.16b	7.88±0.42b
薄荷提取物	23.62±1.35c	3.39±0.04b	2.25±0.11f	2.15±0.49b	10.4±074a

试验处理	多酚/% (冻干样）	黄酮总量/% （冻干样）	木质素/% （冻干样）	氨基酸/% （冻干样）	丙二醛/mg/kg （鲜叶）
对照	19.55±0.36a	3.30±0.01b	2.12±0.12a	1.67±0.35a	9.15±0.00a
冬青油	20.32±0.09a	2.63±0.78a	1.76±0.09b	2.34±0.36c	8.43±0.08a
二氢茉莉酸酯	24.28±0.37c	3.62±0.04b	1.96±0.10c	2.26±0.44bc	7.60±0.72b
迷迭香提取物	21.76±0.10b	2.73±0.33a	2.54±0.12d	2.60±0.19d	6.15±0.30c
紫苏提取物	23.19±0.39c	2.63±0.78a	2.32±0.10e	2.13±0.16b	7.88±0.42b
薄荷提取物	23.62±1.35c	3.39±0.04b	2.25±0.11f	2.15±0.49b	10.4±074a

试验处理	多酚氧化酶 (PPO)	苯丙解氨酶 (PAL)	脂氧合酶 (LOX)	超氧化物歧化酶(SOD)
对照	2.46±0.44a	111.0±9.4b	430.8±22.4a	18.3±0.6a
冬青油	7.25±0.35c	63.7±5.2a	656.7±9.4b	102.7±3.9c
二氢茉莉酸酯	4.64±0.23b	66.3±2.3a	635.0±35.4bc	36.4±0.2b
迷迭香提取物	10.7±0.04d	133.7±1.5c	804.2±38.9d	129.5±7.6d
紫苏提取物	5.24±0.30b	187.1±0.6d	1083.3±40.1e	188.1±7.8e
薄荷提取物	7.64±0.69c	103.6±9.0b	696.7±11.8c	112.3±0.8c

试验处理	多酚氧化酶 (PPO)	苯丙解氨酶 (PAL)	脂氧合酶 (LOX)	超氧化物歧化酶(SOD)
对照	2.46±0.44a	111.0±9.4b	430.8±22.4a	18.3±0.6a
冬青油	7.25±0.35c	63.7±5.2a	656.7±9.4b	102.7±3.9c
二氢茉莉酸酯	4.64±0.23b	66.3±2.3a	635.0±35.4bc	36.4±0.2b
迷迭香提取物	10.7±0.04d	133.7±1.5c	804.2±38.9d	129.5±7.6d
紫苏提取物	5.24±0.30b	187.1±0.6d	1083.3±40.1e	188.1±7.8e
薄荷提取物	7.64±0.69c	103.6±9.0b	696.7±11.8c	112.3±0.8c

试验处理	超氧阴离子自由基	羟自由基
对照	32.67 ±7.96a	55.33 ±1.27a
冬青油	96.19 ± 4.36d	54.02 ± 0.86a
二氢茉莉酸酯	52.81 ± 14.89bc	64.07 ± 2.09b
迷迭香提取物	61.34 ±1.28c	58.03 ± 1.64c
紫苏提取物	43.19 ± 6.93b	57.04 ± 1.05b
薄荷提取物	60.62 ± 6.93c	43.12 ± 2.27b