Application Progress of Double Haploid Breeding Technology in Rice Breeding

doi:10.11924/j.issn.1000-6850.casb2025-0594

Abstract

Abstract:

Doubled Haploid (DH) technology can rapidly achieve genotypic homozygosity., shorten the breeding cycle, and enhance breeding efficiency. It has been widely applied in maize breeding. However, reports on its application in rice variety selection remain relatively limited. This paper reviews the research progress of DH technology in rice, summarizing the processes of haploid induction, chromosome doubling, screening, and identification. It analyzes the applications of this technology in fundamental research areas such as constructing genetic maps, mapping quantitative trait loci (QTLs), editing haploid genes, creating homozygous mutants, and genome sequencing. Additionally, it summarizes the application of DH technology in the development of new rice varieties. To address challenges like plant dwarfism, low survival rates, and unstable induction efficiency encountered during rice breeding using haploid technology, strategies are proposed to optimize the technology, including utilizing diverse germplasm resources for hybridization, gene editing at the haploid stage, and integrating molecular marker-assisted selection, genomic selection, or multi-gene synergistic editing. Finally, this review provides insights into the future development directions of DH breeding in rice, offering a theoretical reference for its application in cultivating new rice varieties.

Key words: rice, double haploid, breeding technology, variety selection, genetic improvement

CLC Number:

S511稻

LI Tang, LI Qianlong, DU Ming, FANG Yu. Application Progress of Double Haploid Breeding Technology in Rice Breeding[J]. Chinese Agricultural Science Bulletin, 2026, 42(17): 1-10.

Figures/Tables 2

References 64

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基因名称	诱导过程	诱导方式	诱导产生单倍体的原因	参考文献
OsMTL/PLA1	卵细胞受精依赖性	孤雌生殖	基因突变，诱导父系基因组消除	[7]
OspPLAγ	卵细胞受精依赖性	孤雌生殖	基因突变，诱导父系基因组消除	[46]
OsPLD	卵细胞受精依赖性	孤雌生殖	基因突变，在磷脂酶单倍体诱导途径中具有协同作用	[48]
OsDMP	卵细胞受精依赖性	不具备独立的单倍体诱导能力	与MTL互作诱导产生单倍体	[49]
OsCENH3	卵细胞受精依赖性	着丝粒介导，孤雌生殖	自身基因组消除，CENH3蛋白功能缺失，与cenh3突变体共同诱导	[41]
OsBBM	卵细胞受精非依赖性	孤雌生殖,无融合生殖	卵细胞异位表达，导致亲本基因组消除	[50-51]

基因名称	诱导过程	诱导方式	诱导产生单倍体的原因	参考文献
OsMTL/PLA1	卵细胞受精依赖性	孤雌生殖	基因突变，诱导父系基因组消除	[7]
OspPLAγ	卵细胞受精依赖性	孤雌生殖	基因突变，诱导父系基因组消除	[46]
OsPLD	卵细胞受精依赖性	孤雌生殖	基因突变，在磷脂酶单倍体诱导途径中具有协同作用	[48]
OsDMP	卵细胞受精依赖性	不具备独立的单倍体诱导能力	与MTL互作诱导产生单倍体	[49]
OsCENH3	卵细胞受精依赖性	着丝粒介导，孤雌生殖	自身基因组消除，CENH3蛋白功能缺失，与cenh3突变体共同诱导	[41]
OsBBM	卵细胞受精非依赖性	孤雌生殖,无融合生殖	卵细胞异位表达，导致亲本基因组消除	[50-51]