Research Progress on Role of Glycine-rich Proteins in Plant Response to Abiotic Stress

doi:10.11924/j.issn.1000-6850.casb2025-0681

Abstract

Abstract:

Glycine-rich proteins (GRPs) are a family of proteins containing a high proportion of Glycine (20%-70%), which are widely present in prokaryotes and eukaryotes and are important regulatory factors for plants to respond to abiotic stresses (high salt, drought, high and low temperatures, etc.). They regulates plant growth, development and stress resistance response through RNA binding, protein interaction and other means. This article reviews the structural characteristics, classification system, biological functions and the mechanism of action under abiotic stress of plant GRPs. The results show that, structurally, all contain GR domains, and different subcategories also contain specific domains such as RNA recognition motif (RRM), cold shock domain (CSD), and CCHC zinc finger. It can be classified into five categories. Among them, category IV (GR-RBPs) contains RNA binding functions and is the most abundant in plants. Functionally, GRPs have obvious tissue specificity and can participate in plant growth and development by promoting cell elongation and regulating stomatal opening and closing. The role of GRPs in the response to abiotic stress is emphasized: under low-temperature stress, AtGRP2 of Arabidopsis thaliana, a GRPs of the Ⅳc subfamily, enhances the plant's frost resistance through RNA chaperone activity. Under high-temperature stress, the expression of OsGRP3/OsGRP162 in rice and BcGRP1 in non-head-forming Chinese cabbage is induced by heat, maintaining the heat tolerance of plants. In salt stress and osmotic stress, the Limonium bicolor LbGRP1 enhances salt tolerance by increasing the activity of antioxidant enzymes. Under drought stress, Arabidopsis AtGRP2/AtGRP7 and rice OsGRP3 enhance drought resistance by regulating stomatal movement, ROS metabolism and lignin synthesis. Finally, the future research directions are discussed, and it is proposed that multi-mics and genetic techniques should be combined to analyze the upstream regulatory network of GRPs and the collaborative response mechanism under multiple stresses, providing a theoretical basis for breeding stress-resistant crop varieties.

Key words: plant, glycine-rich proteins, abiotic stress, growth and development, stress resistance

HUANG Jinli, HUO Jiaohan, QIU Rongwei, LIN Fang, LU Caiyun, XU Weifeng, LIU Jianping. Research Progress on Role of Glycine-rich Proteins in Plant Response to Abiotic Stress[J]. Chinese Agricultural Science Bulletin, 2026, 42(2): 57-64.

Figures/Tables 2

References 46

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基因	研究发现	作用机制
AtGR-RBP5	敲除后根变短、叶变小、花轴变短；超表达株系细胞变长，缺失突变体细胞变短	调控气孔保卫细胞开关参与植物对环境压力的适应性调控
AtGR-RBP7	调节气孔保卫细胞的开与关，参与植物抵御环境胁迫；与AtGR-RBP8相互调节转录	调节气孔开闭适应环境胁迫；与AtGR-RBP8相互调节转录

基因	研究发现	作用机制
AtGR-RBP5	敲除后根变短、叶变小、花轴变短；超表达株系细胞变长，缺失突变体细胞变短	调控气孔保卫细胞开关参与植物对环境压力的适应性调控
AtGR-RBP7	调节气孔保卫细胞的开与关，参与植物抵御环境胁迫；与AtGR-RBP8相互调节转录	调节气孔开闭适应环境胁迫；与AtGR-RBP8相互调节转录

逆境类型		GRPs成员及研究对象			表达变化及功能机制
温度胁迫	低温胁迫	Pa-RRM-GRP1			受3℃冷处理诱导，温度回升后表达恢复，参与冷响应调节
		AtGRP2/AtGRP24/AtGRP27			冷处理下表达上调，过表达AtGRP2增强抗冻性；AtGRP7通过调节气孔运动提升耐冻能力
		BnGRP1			具DNA/RNA解链活性，异源表达于拟南芥可加速冷/冻胁迫下萌发，增强耐冻性
		CsGR-RBP3			低温下转录上调，过表达于拟南芥可降低ROS水平，提高CAT、SOD活性及冷冻存活率。
	高温胁迫	OsGRP3/OsGRP3162			夜间表达达峰，受热胁迫(45℃)诱导，为夜间耐热性必需
		BcGRP1			高温处理6 h后表达持续上调，48 h达峰值，参与耐热响应
		ItGRP			ItGRP1/5/7/9在48~72 h高温下表达显著升高，增强耐热性
		SbGR-RBP			分布于细胞核和细胞质，与CaM互作（Ca²⁺依赖），结合核酸能力受调控，参与热胁迫响应
		半夏GR-RBPs			38℃处理后转录和蛋白水平显著积累，缓解高温诱导的氧化胁迫
盐胁迫和渗透胁迫			LbGRP1	过表达于烟草可提高SOD、CAT活性及脯氨酸含量，降低Na⁺含量和Na⁺/K⁺比值，增强耐盐性
盐胁迫和渗透胁迫			ZjGR-RBP	受NaCl诱导，负调控耐盐性，定位于细胞核和细胞质
干旱胁迫			AtGRP2、AtGRP7	过表达于水稻可提高干旱后存活率及籽粒产量，通过调控气孔和细胞吸水势能增强抗旱性
			MpGR-RBP1	干旱下表达量增加，基因差异可能影响抗旱性强弱
			NtGR-RBPs	NaCl处理使其表达降低；可能通过不依赖ABA的信号通路在逆境信号转导中发挥作用
			OsGRP3	受干旱、盐、ABA 显著诱导；ABA或甘露醇处理后，蛋白由胞质/ 核转移至胞质颗粒状结构，正向调控耐旱性

逆境类型		GRPs成员及研究对象			表达变化及功能机制
温度胁迫	低温胁迫	Pa-RRM-GRP1			受3℃冷处理诱导，温度回升后表达恢复，参与冷响应调节
		AtGRP2/AtGRP24/AtGRP27			冷处理下表达上调，过表达AtGRP2增强抗冻性；AtGRP7通过调节气孔运动提升耐冻能力
		BnGRP1			具DNA/RNA解链活性，异源表达于拟南芥可加速冷/冻胁迫下萌发，增强耐冻性
		CsGR-RBP3			低温下转录上调，过表达于拟南芥可降低ROS水平，提高CAT、SOD活性及冷冻存活率。
	高温胁迫	OsGRP3/OsGRP3162			夜间表达达峰，受热胁迫(45℃)诱导，为夜间耐热性必需
		BcGRP1			高温处理6 h后表达持续上调，48 h达峰值，参与耐热响应
		ItGRP			ItGRP1/5/7/9在48~72 h高温下表达显著升高，增强耐热性
		SbGR-RBP			分布于细胞核和细胞质，与CaM互作（Ca²⁺依赖），结合核酸能力受调控，参与热胁迫响应
		半夏GR-RBPs			38℃处理后转录和蛋白水平显著积累，缓解高温诱导的氧化胁迫
盐胁迫和渗透胁迫			LbGRP1	过表达于烟草可提高SOD、CAT活性及脯氨酸含量，降低Na⁺含量和Na⁺/K⁺比值，增强耐盐性
盐胁迫和渗透胁迫			ZjGR-RBP	受NaCl诱导，负调控耐盐性，定位于细胞核和细胞质
干旱胁迫			AtGRP2、AtGRP7	过表达于水稻可提高干旱后存活率及籽粒产量，通过调控气孔和细胞吸水势能增强抗旱性
			MpGR-RBP1	干旱下表达量增加，基因差异可能影响抗旱性强弱
			NtGR-RBPs	NaCl处理使其表达降低；可能通过不依赖ABA的信号通路在逆境信号转导中发挥作用
			OsGRP3	受干旱、盐、ABA 显著诱导；ABA或甘露醇处理后，蛋白由胞质/ 核转移至胞质颗粒状结构，正向调控耐旱性