Research Progress on Tissue Culture and Genetic Transformation in Chinese Chive

doi:10.11924/j.issn.1000-6850.casb2025-0555

Abstract

Abstract:

To issues of the lag in biological breeding of Chinese chives (Allium tuberosum) and the difficulty in achieving breakthroughs in functional research on many superior genes, there is an urgent need to clarify the current status and future directions of tissue culture and genetic transformation research as the key technological foundation. This paper reviews the domestic and international research achievements over three decades in the field, focusing on key aspects such as explant selection, sterilization optimization, medium screening, plant growth regulator formulation, rooting culture, acclimatization and transplanting, and genetic transformation conditions. It is found that: (1) the current tissue culture of Allium tuberosum remains in the primary stage of biological breeding. The explants are mainly flower axis and root tip, MS medium is commonly used basic medium, and the hormone ratio is mainly ' 6-BA + NAA ' combination. (2) The somatic embryo induction system is weak, the proliferation coefficient is low, the genetic transformation efficiency is insufficient, and there are problems such as non-standard terminology and industrial application faults. (3) Agrobacterium-mediated transformation is currently the main method, but has not yet bred transgenic varieties. In summary, non-standard terminology, imperfect regeneration system and low genetic transformation efficiency are the core bottlenecks restricting the molecular breeding of Allium tuberosum. The authors propose that it is necessary to standardize professional terminology, optimize explant-specific culture conditions by variety, clarify regeneration pathways and summarize the best system, optimize the genetic transformation system, strengthen somatic embryo and genetic transformation synergistic breeding, directionally introduce stress-tolerant and high-quality genes, and surmount the "bottleneck" problem of Allium tuberosum molecular breeding through synergistic innovation of multiple technologies to provide support for technological industrialization.

Key words: Chinese chives, tissue culture, regeneration system, genetic transformation, synergistic breeding

SUN Yaling, ZHANG Mingjun, LIU Qinggang, SHU Rui, WANG Zhenbao, FU Zaiqiu, HUO Yumeng. Research Progress on Tissue Culture and Genetic Transformation in Chinese Chive[J]. Chinese Agricultural Science Bulletin, 2026, 42(2): 71-77.

Figures/Tables 1

References 55

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外植体类型	消毒剂	参考文献
种子	75%乙醇30 s，0.1%升汞和0.1% Tween-20的混合溶液10 min	[25]
种子	75%乙醇30 s，0.1%升汞和0.1% Tween-20的混合溶液10 min	[26]
种子	70%乙醇浸泡5 min，再用0.1%升汞溶液消毒10 min，无菌水冲洗3遍	[22]
种子	70%乙醇浸泡30 s，再用0.1%升汞消毒10 min	[27]
花蕾	洗衣粉溶液清洗试验材料，水冲洗4~5遍，再用0.1%升汞浸泡10 min，75%乙醇30 s	[19]
花托	先用洗洁精和清水洗去表面灰尘，流水冲洗30 min，75%乙醇浸泡30 s，0.2%升汞8 min	[18]

外植体类型	消毒剂	参考文献
种子	75%乙醇30 s，0.1%升汞和0.1% Tween-20的混合溶液10 min	[25]
种子	75%乙醇30 s，0.1%升汞和0.1% Tween-20的混合溶液10 min	[26]
种子	70%乙醇浸泡5 min，再用0.1%升汞溶液消毒10 min，无菌水冲洗3遍	[22]
种子	70%乙醇浸泡30 s，再用0.1%升汞消毒10 min	[27]
花蕾	洗衣粉溶液清洗试验材料，水冲洗4~5遍，再用0.1%升汞浸泡10 min，75%乙醇30 s	[19]
花托	先用洗洁精和清水洗去表面灰尘，流水冲洗30 min，75%乙醇浸泡30 s，0.2%升汞8 min	[18]