Abstract:

This study delves into the crucial factors that influence the survival rate of cotton tissue culture seedlings during transplantation. Addressing the pervasive issue of low survival rates during direct transplantation, we aim to offer a robust and efficient transplantation approach for cotton tissue culture seedlings. Utilizing these seedlings as our primary experimental subject, we examined their survival rate, root system, and growth of stem and leaves under various environmental conditions, substrates, and root system states. The seedlings were rooted within a culture container and subsequently transplanted into a substrate comprising a 1:1 volume ratio of perlite and vermiculite, nourished with a 1/2MS nutrient solution. By maintaining optimal humidity levels during the recovery phase, we observed enhanced seedling vigor, with survival rates soaring from an initial 50% to a remarkable 100%. This method is simple to operate and has a high survival rate, establishing an efficient and stable method for the domestication of cotton tissue culture seedlings. In addition to its affordability, it eliminates the need for grafting, boasts a higher boll-setting rate, and holds promising potential for the burgeoning realm of cotton biotechnology.

Key words: cotton, tissue culture seedling, acclimatization, survival rate, biotechnology