Study on Tissue Culture Technology and Rapid Propagation of Hydrangea paniculata ‘Garden Lace’

doi:10.11924/j.issn.1000-6850.casb2022-0980

Abstract

Abstract:

The stem segments of Hydrangea paniculata ‘Garden Lace’ with axillary buds were used as explants to study the sterilization protocol, the effects of basic culture mediums and plant growth regulators on axillary buds germination, multiple shoot proliferation and adventitious root induction, and the influence of different substrates on the transplanting of tissue culture seedlings. The sterilization effects of NaClO at various chlorine concentrations and treatment times were compared. The treated axillary buds induction, proliferation, and rooting were taken on the basic mediums of MS, 1/2MS, WPM, and 1/2WPM, with different concentrations of 6-BA, NAA, or IBA. The generated tissue culture seedlings were transplanted into various substrates that were mixed with peat, perlite, and vermiculite. The results showed that the most suitable sterilization method was to soak the explant in 75% ethanol for 30 s, followed by 3-4 washings with sterile water. After that, explants were treated with NaClO of 2.0% available chlorine concentration for 8-10 min, followed by 4-6 washings with sterile water. The best start-up medium for axillary buds was 1/2WPM+ 2.0 mg/L 6-BA, for shoots was WPM+ 2.0 mg/L 6-BA+ 0.2 mg/L IBA, and for rooting was 1/2WPM+ 0.3-0.5 mg/L IBA. The best substrate for transplanting the plantlets was an equal amount of well-mixed perlite, vermiculite, and peat. The study developed a quick propagation system for H. paniculata ‘Garden Lace’ through tissue culture, and provided a technical guidance for factory-scale seedling development and mass production.

Key words: Hydrangea paniculata, culture in vitro, rapid propagation technology, tissue culture seedling, explant sterilization, transplant seedling

DENG Yanming, QIN Ziyi, CHEN Shuangshuang, QIU Shuai, FENG Jing, GAO Kai, QI Xiangyu, CHEN Huijie. Study on Tissue Culture Technology and Rapid Propagation of Hydrangea paniculata ‘Garden Lace’[J]. Chinese Agricultural Science Bulletin, 2023, 39(31): 76-82.

Figures/Tables 7

References 25

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处理	萌芽率	处理	萌芽率	处理	萌芽率	处理	萌芽率
1	90.0±2.7b	5	95.6±2.0ab	9	71.1±3.7d	13	20.0±4.0f
2	95.6±2.0ab	6	93.3±2.4b	10	95.6±2.2ab	14	66.7±4.1d
3	72.2±3.8d	7	67.8±3.3d	11	72.2±3.1d	15	80.0±2.9c
4	84.4±3.0c	8	84.4±3.0c	12	46.7±5.7e	16	98.9±1.1a

处理	萌芽率	处理	萌芽率	处理	萌芽率	处理	萌芽率
1	90.0±2.7b	5	95.6±2.0ab	9	71.1±3.7d	13	20.0±4.0f
2	95.6±2.0ab	6	93.3±2.4b	10	95.6±2.2ab	14	66.7±4.1d
3	72.2±3.8d	7	67.8±3.3d	11	72.2±3.1d	15	80.0±2.9c
4	84.4±3.0c	8	84.4±3.0c	12	46.7±5.7e	16	98.9±1.1a

处理	增殖系数	增殖芽生长状态
1	1.5±0.2f	矮小，叶色正常，大量愈伤
2	1.7±0.1f	矮小，叶片发黄，无愈伤
3	1.5±0.2f	矮小，叶片发黄，无愈伤
4	2.7±0.1e	矮小，叶片发黄，有愈伤
5	1.6±0.2f	生长良好，叶片发黄，无愈伤
6	3.1±0.1de	矮小，叶色正常，微量愈伤
7	1.7±0.1f	矮小，叶色正常，有愈伤
8	1.9±0.1f	生长良好，叶色正常，有愈伤
9	4.7±0.2b	矮小，叶片发黄，无愈伤
10	5.8±0.2a	生长良好，叶色正常，无愈伤
11	3.2±0.1d	矮小，叶片发黄，无愈伤
12	2.9±0.1de	生长良好，叶片正常，无愈伤
13	3.9±0.2c	生长良好，叶色正常，有愈伤
14	4.1±0.1c	矮小，叶色正常，无愈伤
15	1.5±0.2f	生长良好，叶色正常，无愈伤
16	4.0±0.2c	生长良好，叶色正常，少量愈伤

处理	增殖系数	增殖芽生长状态
1	1.5±0.2f	矮小，叶色正常，大量愈伤
2	1.7±0.1f	矮小，叶片发黄，无愈伤
3	1.5±0.2f	矮小，叶片发黄，无愈伤
4	2.7±0.1e	矮小，叶片发黄，有愈伤
5	1.6±0.2f	生长良好，叶片发黄，无愈伤
6	3.1±0.1de	矮小，叶色正常，微量愈伤
7	1.7±0.1f	矮小，叶色正常，有愈伤
8	1.9±0.1f	生长良好，叶色正常，有愈伤
9	4.7±0.2b	矮小，叶片发黄，无愈伤
10	5.8±0.2a	生长良好，叶色正常，无愈伤
11	3.2±0.1d	矮小，叶片发黄，无愈伤
12	2.9±0.1de	生长良好，叶片正常，无愈伤
13	3.9±0.2c	生长良好，叶色正常，有愈伤
14	4.1±0.1c	矮小，叶色正常，无愈伤
15	1.5±0.2f	生长良好，叶色正常，无愈伤
16	4.0±0.2c	生长良好，叶色正常，少量愈伤