Abstract: The paper discussed the effect of three kinds of cytokinin: kinetin(KT), benzylaminopurine(BAP) and isopentene group adenine(2ip) on Dioscorea opposite culture in virto. There are two varieties: Chinese yam rhizome and feng yam. Results were as follows: in MS basic medium+KT2mg/L or 2ip, the two varieties growed well. Appending NAA0.2 mg/L could promote radication commonly. But in MS+BAP2 mg/L there was obvious difference from the two varieties. Chinese yam rhizome axillary bud was restrainted. But feng yam was prone to form fascicular bud. When supplemented with NAA0.2 mg.L-1, NAA and BAP could accelerate Chinese yam rhizome axillary bud growth and increase the number of buds. But to feng yam made no difference. From comparison the effect of different media screened out MS+KT1+NAA0.2 mg/L +0.1%activated carbon were appropriate for feng yam.