Abstract:

Tomato cultivars were used as the materials, and cotyledon was cultured to optimize the transformation system via Agrobacterium tumefaciens. The results showed that the highest transformation efficiency was obtained when the explants were preconditioned on preconditioning medium for 2 days prior to infect with Agrobacterium tumefaciens (OD600=0.6) for 6 min growing on the culture medium of MS+2.0 mg/L 6-BA+0.2 mg/L IAA. It was proved that the target NPTII gene had been integrated into the genome of regenerated plants by PCR analysis. This study established a high efficient genetic transformation system of tomato cytoledons by Agrobacterium tumefaciens mediated.