Abstract:

The aim was to analyze the nucleotide and protein sequences of CsCCD7, and provide the theoretical bases to understand the biological functions of unknown proteins. Using a series bioinformatics computer software, database and online programs, the nucleotide and protein sequences of CsCCD7 were analyzed. The results showed that the ORF of CsCCD7 consisted of 1665 bp, and encoded a 554 AA protein which were homologous with carotenoid oxygenase 7; CsCCD7 was located at Chromosome 6 in cucumber, and its full genomic sequence consisted of seven extrons and six introns. CsCCD7 molecular weight was 62.7 KDa and was an unstable protein; there were many phosphorylation sites in CsCCD7. CsCCD7 was non-secretory protein, and had no transmembrane domain. CsCCD7 was located in chloroplast, and belonged to CCD7 evolution branch by phylogenetic analysis. CsCCD7 secondary structure contained several kinds of conformations, and the quantity of β-sheet plus β-turn was more than quantity of α-helix, so this protein was hydrophilicity protein. Both secondary and tetary structures’ prediction results of CsCCD7 showed it was classified into carotenoid oxygenase family. CsCCD7 was the member of CCDs family, and the homologous gene of the gene of inhibiting the axillary bud growth in cucumber. It may play a role in cucumber branching development, strigolactone’s synthesis and signal transduction pathway. The function of CsCCD7 is deserved to study further.