Abstract:

In this paper, different explants were used in the study on tissue culture of Dendrobium officinale, for the purpose of choosing proper propagation ways of development in medicinal herbs. It’s cultured with seed embryo and stem segments as explants in Dendrobium officinale. The results indicated that there were differences between their tissue cultural techniques, such as initial conditions, propagation procedure, propagation coefficient, subculture cycle, rooting differentiation and seedlings transplanting. According to the comparative test, it’s thought that it was more suitable for constructing the tissue cultural system with the stem segments as explants, which is more economical, more efficient and steady. It’ll be the reference of material and technical bases in large scale seedlings production.