Abstract:

Obtaining sufficient quantity and high quality of RNA and DNA is the base for carrying out researches in molecular biology. DNA from Bipolaris sorokiniana was attracted by two cell wall lysis methods using lysozyme and liquid nitrogen grinding methods, respectively. Integrality and purity of nucleic acids were detected with agarose gel electrophoresis, ultraviolet absorption and PCR. Ideal results were obtained, when extracting DNA, high quality RNA was obtained at the same time, by using both methods. The results of PCR indicated that the integrality of DNA was good, The quality of DNA was similar for the two methods used, while the quality of RNA was better when extracted with lysozyme than it with liquid nitrogen grinding. The DNA extraction system of Bipolaris sorokiniana by using lysozyme method was suitable for RNA extraction, it provided a simple, safe and feasible method for extracting both DNA and RNA at the same time from Bipolaris sorokiniana.