Abstract:

In order to provide more gene resources for biological control, identify vip3 gene-types of 100 Bacillus thuringiensis isolates collected from Heilongjiang Province by PCR, perform cloning, expression and bioassay of vip3 gene for cry gene reserves. Identification used universal primer of vip3 gene, perform Cloning, expression and bioassay of full-length gene. The results showed that, LB73 contains vip3Aa gene. The gene full-length sequence was 2370 bp. Coding 789 amino acid residues, MW 88 kD. Registered in GenBank with accession number JQ228436 and was designated vip3Aa46 by International Nomenclature Committee of Bacillus thuringiensis. LC50 of vip3Aa46 against Spodoptera exigua was 4.02 μg/g; LC50 of vip3Aa46 against Helicoverpa armigera was 387.72 μg/g. The results of bioassay showed soluble protein of vip3Aa46 had high activities for Spodoptera exigua and certain activities for Helicoverpa armigera .