Abstract:

To uncover the mechanism of strawberry inoculated with Colletotrichum gloeosporioides and compare gene express difference of the treated and untreated leaves by using the suppression subtractive hybridization (SSH) so as to find the differential express related genes. The total RNA samples of strawberry leaves were prepared and mRNA samples were isolated. cDNA were synthesized and digested with Rsa I. One differential expressing cDNA library was constructed with the restriction enzymes cDNA of inoculated leaves as driver and the restriction enzymes cDNA of untreated as tester. PCR results suggested that the insertion segments were between 100 to 1000 bp and the library was suitable for the following work for isolation the full length of genes. 1500 clones were selected and sequenced, and 1377 unique ESTs were obtained. The ESTs were annotated by similarity to orthologs and paralogs detected with Blast in NCBI database. Some of the ESTs might be related closely with stress response, signal transduction, protein synthesis, protein-protein interaction, metabolism, transmembrane, transcription factor, metal transporter or unknown. This study lays a foundation for the further study on isolation related genes in strawberry.