Abstract:

A Bacillus thuringiensis strain which produced crystal was isolated from soil with the method of sodium acetate-antibiotics heating in this study. By the known cry1 gene sequences, a pair of primers was designed. The PCR amplification was completed with the template of the DNA of the isolated strain, and the cloned fragment was sequenced. The results of sequence analysis indicated that this fragment had at least 90%-99% homology compared with the other cry1 genes. The target fragment was ligated with pBI121 vector and the plant expression vector was constructed, which was transferred into the cotyledon node system in soybean by Agrobacterium tumefaciens-mediated method and severa1 transformed plants were obtained which tested by PCR amplification. This result indicated that the transformed system in soybean was established.