Abstract:

Recycling DNA fragments from polyacrylamide gel is a common technique in molecular biology, especially for recycling DNA short fragments. In this experiment, two methods (boiling method and water-bath method) were used to extract short DNA fragments which were co-precipitated with absolute ethanol and 3 mol/L sodium acetate together. The results were compared by polyacrylamide gel electrophoresis. At last, short DNA fragments was analyzed by the method of ultraviolet absorption. It showed that the recovery rate of short DNA fragments in water-bath method was higher than boiling method. Water-bath method combined with ethanol precipitation for extracted short DNA fragments had many advantages, such as economical and convenient which should be advocated to use widespread.