Abstract:

In this study, the core primers were selected from 20 pairs of SSR primers to identify the authenticity of maize varieties in Liaoning province. PCR amplified products of 10 maize hybrids were detected by 20 basic primer pairs averagely distributed on 10 chromosomes of maize by using SSR markers. 14 pairs of core primers were selected to identify the authenticity of maize varieties on electrophoresis maps and the stability,et al.Among all the core primer pairs, bnlg161k8 was the best on polymorphism and the authenticity of 10 maize varieties was identified only by 3 pairs of primers (bnlg161k8, bnlg2305k4, umc1705w1). 3 pairs of primers (bnlg161k8, bnlg2305k4, umc1705w1) could be the preferred primers by costing savings and improving efficiency.